Secretory pathways of neuropeptides in rat lumbar dorsal root ganglion neurons and effects of peripheral axotomy

The Journal of Comparative Neurology
X ZhangT Hökfelt

Abstract

Using immunocytochemistry combined with confocal and electron microscopy, the secretory pathways related to substance P (SP), calcitonin gene-related peptide (CGRP), galanin (GAL), and neuropeptide Y (NPY) were investigated in neurons in rat lumbar (L) 4 and L5 dorsal root ganglia (DRGs) before and after peripheral axotomy. All four peptides were processed through the regulated secretory pathway in many small neurons in normal DRGs, and CGRP through this pathway also in some large neurons. In many small neurons, two neuropeptides could be sorted into the same or separate large dense-core vesicles (LDCVs). The LDCVs had a significantly larger diameter in small as compared to large DRG neurons. Fourteen days after sciatic nerve cut, the levels of SP- and CGRP-like immunoreactivities (-LIs) and the number of LDCVs containing these peptides were markedly reduced, but SP- and CGRP-LIs were still seen in the regulated pathway. GAL-LI was markedly increased in many small neurons and some large neurons and NPY-LI mainly in large neurons. Both peptides were particularly abundant in the Golgi region. In small neurons, the number of LDCVs containing GAL- or NPY-LI was increased, but did not appear to reach the numbers containing SP- or CG...Continue Reading

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Citations

Dec 10, 1999·Journal of the Autonomic Nervous System·F A Abdulla, P A Smith
Aug 17, 2001·Trends in Neurosciences·R E Zigmond
Aug 17, 2005·Proceedings of the National Academy of Sciences of the United States of America·Kunfu OuyangHeping Cheng
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Hua-Sheng XiaoXu Zhang
Jan 20, 2007·Peptides·Tomas HökfeltMarcelo Villar
Feb 5, 2005·Molecular and Cellular Neurosciences·Natalie J GardinerCharles H Streuli
Nov 9, 2004·Journal of Chemical Neuroanatomy·Eugenia KuteevaTomas Hökfelt
Aug 21, 2015·Molecular and Cellular Neurosciences·Niall KerrDavid Wynick
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Apr 22, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Marc LandryAndré Calas
May 9, 2006·The Journal of Comparative Neurology·Wendy WeissnerGeoffrey M Bove
Oct 17, 2008·Physiological Reviews·Heping Cheng, W J Lederer
Jul 27, 2007·The Journal of Pharmacology and Experimental Therapeutics·Xiao-Hui SunHui-Bi Xu
Aug 2, 2013·American Journal of Physiology. Cell Physiology·Liang-Hong ZhengZhuan Zhou
Sep 2, 2021·The Journal of Comparative Neurology·Hayate JavedSafa Shehab

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