Secretory products of breast cancer cells upregulate hyaluronan production in a human osteoblast cell line.

Clinical & Experimental Metastasis
Nandita Bose, Anna M Masellis

Abstract

Hyaluronan (HA) has been implicated in breast cancer progression and metastasis to lymph nodes. Although breast cancer has a strong propensity for metastasis to the bone, the role of HA in the development of bone metastasis in breast cancer is not well delineated. In order to determine the role of HA in breast cancer induced osteolysis, we examined the effect of secretory products in the conditioned medium of breast cancer cell lines on the expression of hyaluronan synthases (HAS), accumulation of HA in pericellular matrix, secretion of HA in the culture media, and on expression of surface HA receptors, in a human immature osteoblast cell line (Hfob). Our results show that conditioned medium derived from breast cancer cells upregulate the expression of hyaluronan synthases, HAS1 and HAS2, followed by significant increase in pericellular and secreted HA in Hfob cells. Our results further demonstrate that both CD44 and receptor for hyaluronan-mediated motility (RHAMM) are involved in binding cell surface associated HA on Hfob cells. Analysis of the growth factors in the conditioned medium implicates TGF-beta1 in the modulation of HAS1 and HAS2, as well as in the increase in pericellular and secreted HA. This report is the first t...Continue Reading

References

Jan 1, 1981·Calcified Tissue International·G A Rodan, T J Martin
Oct 1, 1995·Endocrine Reviews·R B Dickson, M E Lippman
Apr 1, 1996·Physiological Reviews·G S SteinM Montecino
Nov 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·H Nakamura, H Ozawa
Nov 14, 1997·International Journal of Cancer. Journal International Du Cancer·P K AuvinenV M Kosma
Aug 24, 1999·The Journal of Biological Chemistry·N ItanoK Kimata
Sep 28, 1999·Endocrinology·R J ThomasM T Gillespie
Mar 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Naoki ItanoKoji Kimata
Aug 7, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K SuzukiJ Sodek
Sep 18, 2002·The Journal of Cell Biology·Paola SpessottoValter Gattei
Feb 20, 2003·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Mario WernickeAna Alcestes Morandi
Mar 26, 2003·FEBS Letters·Yuko FujiiYoshiya Tanaka
Oct 21, 2003·International Journal of Cancer. Journal International Du Cancer·Kelli M BullardJames B McCarthy
Jan 27, 2004·Journal of Cellular Biochemistry·Andrea M MastroKristin Guttridge
May 1, 2004·BMC Musculoskeletal Disorders·Charles W Prince
Sep 25, 2004·Clinical & Experimental Metastasis·Qin Yu, Ivan Stamenkovic
Nov 10, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Petri BonoHeikki Joensuu
Dec 28, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Jay J CaoBernard P Halloran
Jan 28, 2005·Clinical & Experimental Metastasis·Robyn R MercerAndrea M Mastro
Feb 22, 2005·The Journal of Biological Chemistry·Katri SaavalainenCarsten Carlberg
Mar 15, 2005·Connective Tissue Research·Takeshi Oguchi, Naoki Ishiguro
Oct 8, 2005·Cancer Treatment and Research·Virginia Kaklamani, Boris Pasche

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Citations

Jan 20, 2015·Journal of Cellular and Molecular Medicine·Katarzyna KamińskaAnna M Czarnecka
Feb 27, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Neil Borg, Michael Holland
Jan 30, 2008·Applied Immunohistochemistry & Molecular Morphology : AIMM·Alaa AfifyAugusto F Paulino

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