Secretory vesicle budding from the trans-Golgi network is mediated by phosphatidic acid levels.

The Journal of Biological Chemistry
A Siddhanta, D Shields

Abstract

Phospholipid metabolism plays a central role in regulating vesicular traffic in the secretory pathway. In mammalian cells, activation of a Golgi-associated phospholipase D activity by ADP-ribosylation factor results in hydrolysis of phosphatidylcholine to phosphatidic acid (PA). This reaction has been proposed to stimulate nascent secretory vesicle budding from the trans-Golgi network. It is unclear whether PA itself or diacylglycerol (DAG), a metabolite implicated in yeast secretory vesicle formation, regulates budding. To distinguish between these possibilities we have used a permeabilized cell system supplemented with phospholipid-modifying enzymes that generate either DAG or PA. The data demonstrate that in mammalian cells accumulation of PA rather than DAG is a key step in regulating budding of secretory vesicles from the trans-Golgi network.

References

May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·N T KtistakisM G Roth
Jan 28, 1994·Science·S CockcroftJ J Hsuan
Mar 15, 1996·Science·P De CamilliP Novick
Jun 20, 1997·The Journal of Biological Chemistry·J H Exton
Jul 1, 1997·Trends in Cell Biology·T F Martin

❮ Previous
Next ❯

Citations

Sep 1, 2001·Cellular and Molecular Life Sciences : CMLS·F GuG Thomas
May 26, 2012·Naunyn-Schmiedeberg's Archives of Pharmacology·Tomoyuki NakanoIchiro Wakabayashi
Jun 30, 2005·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Ana Maria CalderoniMaría Sofía Giménez
Jun 24, 2004·Cellular Signalling·Bai LuoMatthew K Topham
Sep 26, 2003·Trends in Cell Biology·Zachary FreybergDennis Shields
Jun 8, 1999·Chemistry and Physics of Lipids·H KanohI Wada
May 29, 2000·Current Opinion in Genetics & Development·H R de SilvaN W Wood
May 14, 1999·Trends in Cell Biology·M G Roth
Apr 27, 2000·Trends in Biochemical Sciences·G OdorizziS D Emr
Jul 30, 1999·Biochimica Et Biophysica Acta·J H Exton
Jul 30, 1999·Biochimica Et Biophysica Acta·K OhguchiY Nozawa
Jul 30, 1999·Biochimica Et Biophysica Acta·M LiscovitchX Tang
Sep 23, 2011·Chemical Reviews·Paige E SelvyH Alex Brown
Jan 22, 2000·The Biochemical Journal·M LiscovitchX Tang
Aug 4, 2001·The Biochemical Journal·M LocatiS Sozzani
Dec 4, 2002·Immunology·Youngkyun LeeMyung-Un Choi
Sep 12, 2009·The Journal of Biological Chemistry·Michael LungRichard M Epand
Apr 16, 2002·Molecular Biology of the Cell·Dale J PownerMichael J O Wakelam
Jan 26, 2002·Molecular Biology of the Cell·Hisao NagayaHideo Kanoh
Apr 11, 2001·Molecular Biology of the Cell·Z FreybergD Shields
Jul 28, 2006·Molecular Biology of the Cell·Morten K LarsenJens Knudsen
Jun 15, 2007·Molecular Biology of the Cell·Inés Fernández-UlibarriGustavo Egea
Sep 21, 2007·Molecular Biology of the Cell·Francesco FerraroRichard E Mains
May 22, 2001·Molecular and Cellular Biology·Y Kam, J H Exton
Oct 14, 2000·Annual Review of Cell and Developmental Biology·M R Blatt
Aug 8, 2013·Cellular and Molecular Life Sciences : CMLS·Simona KavaliauskieneKirsten Sandvig
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·M StamnesJ E Rothman
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·Z XieV A Bankaitis
Feb 18, 2016·Scientific Reports·Yan-Bing ZhuDong-Min Yin
Oct 29, 2002·FEBS Letters·Bradley T AndresenGuillermo Romero
Jan 11, 2012·Biochimica Et Biophysica Acta·Michal A SurmaKai Simons
Apr 9, 2016·Cellular Logistics·Shuwei XieSteve Caplan
Nov 8, 2015·Pharmacology & Therapeutics·Maryam DarabiAnatol Kontush
Apr 21, 2009·Biochimica Et Biophysica Acta·Christian RiebelingDennis Shields
Jun 25, 2008·Biochimica Et Biophysica Acta·Christian RiebelingDennis Shields
Mar 11, 2005·Journal of Cellular Biochemistry·Masami Hiroyama, John H Exton
Mar 27, 2003·Traffic·Edgar E KooijmanKoert N J Burger

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.