Sec/SRP-independent insertion of two thylakoid membrane proteins bearing cleavable signal peptides

FEBS Letters
S J KimA Mant

Abstract

Two imported thylakoid membrane proteins, PSII-X and PSII-W, are synthesised with cleavable N-terminal signal peptides that closely resemble those of Sec-dependent lumenal proteins. In this report we have reconstituted the insertion of pre-PSII-X and pre-PSII-W into isolated thylakoids. We show that insertion does not require either nucleoside triphosphates or stromal extracts, both of which are required for Sec- and signal recognition particle (SRP)-dependent targeting mechanisms. Insertion is furthermore unaffected by protease treatments that destroy the known protein translocation apparatus in the thylakoid membrane. We conclude that these membrane proteins are inserted by an unusual Sec/SRP-independent mechanism that probably resembles that used by CFoII, and we discuss possible parallels with the biogenesis of phage M13 procoat.

References

Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·Z J LorkovićR Oelmüller
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·X LiN E Hoffman
May 12, 1997·FEBS Letters·J W De GierJ Luirink

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Citations

Mar 24, 1999·Trends in Biochemical Sciences·R E Dalbey, C Robinson
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·D MichlR B Klösgen
Jun 27, 2002·European Journal of Biochemistry·Christophe TissierColin Robinson
Oct 14, 2000·Annual Review of Cell and Developmental Biology·R E Dalbey, A Kuhn
Aug 19, 2007·Biological Chemistry·Danja Schünemann
Feb 12, 2004·Biochimica Et Biophysica Acta·Lan-Xin Shi, Wolfgang P Schröder
Dec 29, 2004·Current Biology : CB·Paul Jarvis, Colin Robinson
Dec 14, 2005·Journal of Molecular Biology·Bo HouRalf Bernd Klösgen
Oct 11, 2016·Molecular Genetics and Genomics : MGG·Longxin WangDeqiang Zhang
Jan 3, 2006·Journal of Plant Physiology·Michael GutensohnRalf Bernd Klösgen
Aug 7, 2003·Molecular & Cellular Proteomics : MCP·Stephen M GómezJulian P Whitelegge
Aug 12, 2000·Journal of Experimental Botany·C RobinsonW Edwards
Jun 22, 2007·The Journal of Biological Chemistry·Stefan Frielingsdorf, Ralf Bernd Klösgen
Aug 29, 2001·The Journal of Biological Chemistry·C A WoolheadC Robinson
Dec 26, 2001·Biochimica Et Biophysica Acta·E Schleiff, R B Klösgen
Apr 27, 1999·Biochimica Et Biophysica Acta·F A WollmanR Nechushtai

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