PMID: 9642191Jun 27, 1998Paper

Sed1p is a major cell wall protein of Saccharomyces cerevisiae in the stationary phase and is involved in lytic enzyme resistance

Journal of Bacteriology
H ShimoiKiyoshi Ito

Abstract

A 260-kDa structural cell wall protein was purified from sodium dodecyl sulfate-treated cell walls of Saccharomyces cerevisiae by incubation with Rarobacter faecitabidus protease I, which is a yeast-lytic enzyme. Amino acid sequence analysis revealed that this protein is the product of the SED1 gene. SED1 was formerly identified as a multicopy suppressor of erd2, which encodes a protein involved in retrieval of luminal endoplasmic reticulum proteins from the secretory pathway. Sed1p is very rich in threonine and serine and, like other structural cell wall proteins, contains a putative signal sequence for the addition of a glycosylphosphatidylinositol anchor. However, the fact that Sed1p, unlike other cell wall proteins, has six cysteines and seven putative N-glycosylation sites suggests that Sed1p belongs to a new family of cell wall proteins. Epitope-tagged Sed1p was detected in a beta-1,3-glucanase extract of cell walls by immunoblot analysis, suggesting that Sed1p is a glucanase-extractable cell wall protein. The expression of Sed1p mRNa increased in the stationary phase and was accompanied by an increase in the Sed1p content of cell walls. Disruption of SED1 had no effect on exponentially growing cells but made stationary-p...Continue Reading

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Citations

Mar 28, 2002·Applied and Environmental Microbiology·Hitoshi ShimoiKiyoshi Ito
Apr 20, 2004·FEMS Yeast Research·Suzanne Bowen, Alan E Wheals
Feb 25, 2006·Yeast·Frans M KlisPiet W J De Groot
Jun 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Guillaume Lesage, Howard Bussey
Dec 1, 2007·The Journal of Biological Chemistry·Sandhya ShankarnarayanJan S Fassler
Mar 23, 2011·Yeast·Lucía BlascoTomás G Villa
Jun 11, 2011·FEMS Microbiology Reviews·Claudio De Virgilio
Jun 13, 2015·G3 : Genes - Genomes - Genetics·Lihong LiLinda L Breeden
Oct 31, 2002·Applied and Environmental Microbiology·Ilaria MannazzuFrancesca Clementi
Oct 31, 2003·Bioscience, Biotechnology, and Biochemistry·Kazuki NagamineKiyoshi Ito
Jan 1, 2010·Yeast·Frans M KlisPiet W J De Groot
Jul 28, 2009·Applied and Environmental Microbiology·Miguel C TeixeiraIsabel Sá-Correia
May 23, 2012·Cellular & Molecular Biology Letters·Fernando CardonaAgustín Aranda
Jan 15, 2016·Molecular & Cellular Proteomics : MCP·Patrick NeubertSabine Strahl
Sep 26, 2006·Applied and Environmental Microbiology·Hong WuHitoshi Shimoi
Aug 29, 2006·Applied and Environmental Microbiology·Yoh-Ichi ShimmaYoshifumi Jigami
Jan 6, 2009·Applied Microbiology and Biotechnology·Kouichi KurodaMitsuyoshi Ueda
Oct 12, 2014·Applied and Environmental Microbiology·Jungu BaeMitsuyoshi Ueda
Aug 22, 2016·Current Genetics·Shawna Miles, Linda Breeden
Aug 8, 2002·FEMS Microbiology Reviews·Frans M KlisStanley Brul
Dec 26, 2006·Applied and Environmental Microbiology·Alane E Wentz, Eric V Shusta
Jan 20, 2009·Molecular Genetics and Genomics : MGG·Jacques H Daniel

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