Sedimentation method for preparation of postoperatively salvaged unwashed shed blood in orthopaedic surgery

British Journal of Anaesthesia
Manuel MuñozD Thomas

Abstract

Salvage and return of unwashed shed blood (USB) after total knee replacement (TKR) is an established blood-saving technique, but some authors question its efficacy and safety and suggest that the shed blood be washed before returning. We evaluated a colloid-based sedimentation method for improving and standardizing the quality of USB collected after TKR without the need for washing. Experiments were performed to find the optimal colloid dose and sedimentation time using diluted donated venous blood. USB samples (n=52) were drawn from the reinfusion bag and mixed with hydroxyethyl starch or gelatine solutions (15-30%, colloid solution volume/total volume × 100). USB red blood cells (RBCs) were allowed to settle by gravity for 30 min, supernatant was evacuated from the syringe, and RBC concentrate was analysed. RBC recovery and other blood cell and chemical removal were calculated according to changes in USB volume and haematocrit. Twenty-five samples from leucodepleted packed RBCs were analysed as a comparator group. Mean haemoglobin (Hb) of USB was 10.9 g dl(-1). After colloid treatment, 90% of RBCs were recovered, and USB Hb was similar to that of leucodepleted packed RBCs (n=25) (18.9 vs 19.6 g dl(-1), respectively; P=NS). In...Continue Reading

References

Nov 22, 1985·Biochimica Et Biophysica Acta·N Maeda, T Shiga
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·P RubinsteinC E Stevens
Jul 27, 1999·The Annals of Thoracic Surgery·W ReentsO Elert
Sep 29, 2001·British Journal of Anaesthesia·D ThomasH Hutchings
Apr 10, 2004·Journal of Cardiothoracic and Vascular Anesthesia·Biao DaiC David Mazer
May 13, 2004·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·M MuñozC Sebastián
Oct 16, 2004·British Journal of Anaesthesia·C Madjdpour, D R Spahn
Dec 9, 2004·Transfusion·Ernil Hansen, Michael Pawlik Hansen
Jun 14, 2005·Journal of Clinical Anesthesia·Hans-Jürgen DieterichHolger K Eltzschig
Feb 27, 2007·Transfusion·Jonathan H Waters, Robert M Dyga
Mar 29, 2008·British Journal of Anaesthesia·P BerisP Van der Linden
Sep 4, 2008·Transfusion·Thomas VolkChristian von Heymann
Jun 11, 2009·Anesthesiology·Martin WestphalHugo Van Aken

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Citations

Nov 7, 2012·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·R EshuisA de Gast

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