Sedolisins, a new class of secreted proteases from Aspergillus fumigatus with endoprotease or tripeptidyl-peptidase activity at acidic pHs

Applied and Environmental Microbiology
Utz ReichardM Monod

Abstract

The secreted proteolytic activity of Aspergillus fumigatus is of potential importance as a virulence factor and in the industrial hydrolysis of protein sources. The A. fumigatus genome contains sequences that could encode a five-member gene family that produces proteases in the sedolisin family (MEROPS S53). Four putative secreted sedolisins with a predicted 17- to 20-amino-acid signal sequence were identified and termed SedA to SedD. SedA produced heterologously in Pichia pastoris was an acidic endoprotease. Heterologously produced SedB, SedC, and SedD were tripeptidyl-peptidases (TPP) with a common specificity for tripeptide-p-nitroanilide substrates at acidic pHs. Purified SedB hydrolyzed the peptide Ala-Pro-Gly-Asp-Arg-Ile-Tyr-Val-His-Pro-Phe to Arg-Pro-Gly, Asp-Arg-Ile, and Tyr-Val-His-Pro-Phe, thereby confirming TPP activity of the enzyme. SedB, SedC, and SedD were detected by Western blotting in culture supernatants of A. fumigatus grown in a medium containing hemoglobin as the sole nitrogen source. A degradation product of SedA also was observed. A search for genes encoding sedolisin homologues in other fungal genomes indicates that sedolisin gene families are widespread among filamentous ascomycetes.

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Citations

Dec 25, 2008·Applied Microbiology and Biotechnology·Jaewoo YoonKatsuhiko Kitamoto
Sep 13, 2011·Journal of Agricultural and Food Chemistry·Jia-Shin LinChao-Hung Kao
May 18, 2010·Applied and Environmental Microbiology·Karine SalaminMichel Monod
Sep 25, 2007·Eukaryotic Cell·Tobias M Hohl, Marta Feldmesser
Aug 19, 2009·Proteomics·Rafael Prados-RosalesAntonio Di Pietro
May 23, 2012·The Journal of Allergy and Clinical Immunology·Laurence MillonMichel Monod
May 10, 2011·International Journal of Medical Microbiology : IJMM·Christian B FleckMatthias Brock
Dec 5, 2015·Frontiers in Plant Science·Rohan G T LoweMarilyn Anderson
Sep 16, 2008·Mycoses·Utz ReichardMichel Monod
Dec 13, 2006·FEMS Microbiology Letters·Karin GiddeyMichel Monod
Jun 12, 2016·Microbial Cell Factories·Christopher P LandowskiMarkku Saloheimo
Aug 19, 2016·Frontiers in Plant Science·Carina FélixAna C Esteves
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Aug 21, 2021·Journal of Industrial Microbiology & Biotechnology·Victoria L KornJoan W Bennett

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