Seed culture and its effect on the growth and lactic acid production of Lactobacillus helveticus

The Journal of General and Applied Microbiology
Abdeltif Amrane

Abstract

Effects of seed culture medium on the subsequent culture of L. helveticus growing on whey supplemented with yeast extract and peptones have been examined. Cells were inoculated when the maximum cellular activity was achieved, i.e. the maximum for the target function, the product of the specific growth rate with the undissociated lactic acid concentration. This function decreased when the nitrogen supplementation of the preculture medium was lowered, resulting only in an increase of the lag phase length of the culture, corresponding to a cellular adaptation to the new medium. On the contrary, when cells were not in the same physiological state, growth and production parameters were affected, maximum rates, as well as maximum biomass concentration. This occurred in case of the comparison of inocula carried out under pH control or not, or when various preculture lengths were tested.

References

Sep 1, 1987·Applied and Environmental Microbiology·G R Bender, R E Marquis
Jan 1, 1996·Advances in Applied Microbiology·J H Litchfield
Feb 26, 2000·Enzyme and Microbial Technology·K Hofvendahl, B Hahn-Hägerdal
Jul 1, 1990·Applied and Environmental Microbiology·L C McDonaldH M Hassan

Citations

Dec 16, 2011·Critical Reviews in Biotechnology·P S Panesar, J F Kennedy

Related Concepts

Whey Proteins
Cell Division Phases
Fermentation
Hydrogen-Ion Concentration
Lactobacillus
Milk Proteins
Fermentors
Ammonium lactate

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