Seed dormancy and ABA metabolism in Arabidopsis and barley: the role of ABA 8'-hydroxylase

The Plant Journal : for Cell and Molecular Biology
Anthony A MillarFrank Gubler

Abstract

We have investigated the relationship between seed dormancy and abscisic acid (ABA) metabolism in the monocot barley and the dicot Arabidopsis. Whether dormant (D) or non-dormant (ND), dry seed of Arabidopsis and embryos of dry barley grains all had similarly high levels of ABA. ABA levels decreased rapidly upon imbibition, although they fell further in ND than in D. Gene expression profiles were determined in Arabidopsis for key ABA biosynthetic [the 9-cis epoxycarotenoid dioxygenasegene family] and ABA catabolic [the ABA 8'-hydroxylase gene family (CYP707A)] genes. Of these, only the AtCYP707A2 gene was differentially expressed between D and ND seeds, being expressed to a much higher level in ND seeds. Similarly, a barley CYP707 homologue, (HvABA8'OH-1) was expressed to a much higher level in embryos from ND grains than from D grains. Consistent with this, in situ hybridization studies showed HvABA8'OH-1 mRNA expression was stronger in embryos from ND grains. Surprisingly, the signal was confined in the coleorhiza, suggesting that this tissue plays a key role in dormancy release. Constitutive expression of a CYP707A gene in transgenic Arabidopsis resulted in decreased ABA content in mature dry seeds and a much shorter after-r...Continue Reading

References

Jan 1, 1996·Methods in Enzymology·D PomponP Urban
Oct 1, 1996·The Plant Journal : for Cell and Molecular Biology·K M Léon-KloosterzielM Koornneef
Jun 20, 1997·Science·S H SchwartzD R McCarty
Nov 10, 1998·Plant Physiology·J E KrochkoA J Cutler
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Jun 25, 1999·Plant Molecular Biology·A FreyA Marion-Poll
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·X Qin, J A Zeevaart
Aug 6, 2000·The Plant Journal : for Cell and Molecular Biology·A J ThompsonI B Taylor
Jan 15, 2002·Current Opinion in Plant Biology·Maarten KoornneefHenk Hilhorst
Jul 1, 1997·The Plant Cell·J. D. Bewley
Jun 17, 2003·Genetics·Carlos Alonso-BlancoMaarten Koornneef
Sep 25, 2003·Annual Review of Plant Biology·Mary A Schuler, Daniele Werck-Reichhart
Dec 13, 2003·Plant Physiology·Rong ZhouSuzanne R Abrams
Mar 9, 2005·Current Opinion in Plant Biology·Frank GublerJohn V Jacobsen
May 3, 2005·Annual Review of Plant Biology·Eiji Nambara, Annie Marion-Poll

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Citations

Dec 6, 2012·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Elizabeth C SchrammCamille M Steber
Jul 21, 2011·Protoplasma·David SeungJan Marc
Jul 20, 2010·Archives of Biochemistry and Biophysics·Ratnakar Vallabhaneni, Eleanore T Wurtzel
Jun 2, 2009·Proceedings of the National Academy of Sciences of the United States of America·Chanhong KimKlaus Apel
Oct 20, 2010·Proceedings of the National Academy of Sciences of the United States of America·Keun Pyo LeeLuis Lopez-Molina
Dec 21, 2011·Omics : a Journal of Integrative Biology·Khushwant SinghAshwani Pareek
Jun 3, 2009·Journal of Experimental Botany·Danica E GogginStephen B Powles
Dec 14, 2011·Journal of Experimental Botany·Paloma Rueda-RomeroLuis Oñate-Sánchez
Jan 28, 2012·Journal of Experimental Botany·Shiling JiangRobert D Hill
Aug 4, 2012·Journal of Experimental Botany·Emmanuel GendreauFrançoise Corbineau
Jul 17, 2009·Proceedings. Biological Sciences·Steven Penfield, John King
Feb 9, 2008·Annual Review of Plant Biology·Ruth FinkelsteinCamille Steber
Jan 22, 2014·Virus Genes·Shunmugiah Veluchamy RameshSyed Masroor Husain
Apr 28, 2006·FEBS Letters·Rogerio MargisPeter M Waterhouse
Jan 22, 2010·The Plant Journal : for Cell and Molecular Biology·Masanori OkamotoMotoaki Seki

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