Seed Transmission of Soybean vein necrosis virus: The First Tospovirus Implicated in Seed Transmission

PloS One
Carol GrovesDamon L Smith

Abstract

Soybean vein necrosis virus (SVNV; genus Tospovirus; Family Bunyaviridae) is a negative-sense single-stranded RNA virus that has been detected across the United States and in Ontario, Canada. In 2013, a seed lot of a commercial soybean variety (Glycine max) with a high percentage of discolored, deformed and undersized seed was obtained. A random sample of this seed was planted in a growth room under standard conditions. Germination was greater than 90% and the resulting seedlings looked normal. Four composite samples of six plants each were tested by reverse transcription polymerase chain reaction (RT-PCR) using published primers complimentary to the S genomic segment of SVNV. Two composite leaflet samples retrieved from seedlings yielded amplicons with a size and sequence predictive of SVNV. Additional testing of twelve arbitrarily selected individual plants resulted in the identification of two SVNV positive plants. Experiments were repeated by growing seedlings from the same seed lot in an isolated room inside a thrips-proof cage to further eliminate any external source of infection. Also, increased care was taken to reduce any possible PCR contamination. Three positive plants out of forty-eight were found using these measur...Continue Reading

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Citations

Apr 23, 2019·Plant Disease·Gustavo A Díaz-CruzBryan J Cassone
Aug 21, 2018·Journal of Economic Entomology·Anitha ChitturiAlana L Jacobson
Sep 1, 2016·Annual Review of Virology·J E Oliver, A E Whitfield
Apr 4, 2019·Frontiers in Microbiology·Jinlong HanPunya Nachappa
Mar 6, 2018·Environmental Entomology·Stacy KeoughPunya Nachappa
Dec 14, 2018·Virus Genes·Jing Zhou, Ioannis E Tzanetakis
Feb 1, 2017·Functional Plant Biology : FPB·Mariadaniela LópezMaría Luisa Izaguirre-Mayoral

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Datasets Mentioned

BETA
HQ728385.1
HQ728386.1
HQ728387.1

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
PCR
Reverse
electrophoresis
ELISA
RNA-seq

Software Mentioned

Bowtie short
BLASTn
PrimerQuest

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