Seeded Amplification of Chronic Wasting Disease Prions in Nasal Brushings and Recto-anal Mucosa-Associated Lymphoid Tissues from Elk by Real-Time Quaking-Induced Conversion

Journal of Clinical Microbiology
Nicholas J HaleyJürgen A Richt

Abstract

Chronic wasting disease (CWD), a transmissible spongiform encephalopathy of cervids, was first documented nearly 50 years ago in Colorado and Wyoming and has since been detected across North America and the Republic of Korea. The expansion of this disease makes the development of sensitive diagnostic assays and antemortem sampling techniques crucial for the mitigation of its spread; this is especially true in cases of relocation/reintroduction or prevalence studies of large or protected herds, where depopulation may be contraindicated. This study evaluated the sensitivity of the real-time quaking-induced conversion (RT-QuIC) assay of recto-anal mucosa-associated lymphoid tissue (RAMALT) biopsy specimens and nasal brushings collected antemortem. These findings were compared to results of immunohistochemistry (IHC) analysis of ante- and postmortem samples. RAMALT samples were collected from populations of farmed and free-ranging Rocky Mountain elk (Cervus elaphus nelsoni;n= 323), and nasal brush samples were collected from a subpopulation of these animals (n= 205). We hypothesized that the sensitivity of RT-QuIC would be comparable to that of IHC analysis of RAMALT and would correspond to that of IHC analysis of postmortem tissue...Continue Reading

References

Oct 1, 1982·Journal of Wildlife Diseases·E S Williams, S Young
Jan 1, 1980·Journal of Wildlife Diseases·E S Williams, S Young
May 1, 1996·Journal of Clinical Microbiology·L J van KeulenJ P Langeveld
Sep 2, 2000·Journal of Clinical Microbiology·K I O'RourkeD P Knowles
Nov 21, 2000·Journal of Wildlife Diseases·M W MillerE T Thorne
Nov 29, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S O DebeerA A Bencsik
Feb 21, 2003·The New England Journal of Medicine·Gianluigi ZanussoSalvatore Monaco
Apr 12, 2003·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·T R SprakerB E Powers
Aug 16, 2003·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Charles P HiblerBarbara E Powers
Feb 3, 2004·Annals of Neurology·Massimo TabatonGianluigi Zanusso
Jun 23, 2004·Emerging Infectious Diseases·John A PainterRobert V Tauxe
Mar 3, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jiri G SafarStanley B Prusiner
Sep 6, 2005·The Journal of Veterinary Medical Science·Tae-Yung KimYong-Soon Lee
Sep 20, 2005·Lancet Neurology·Richard T Johnson
Apr 14, 2006·Journal of Virology·Crista DeJoiaRichard A Bessen
Oct 7, 2006·Science·Candace K MathiasonEdward A Hoover
Nov 24, 2006·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Terry R SprakerRandy D Munger
Nov 17, 2007·Neuroreport·Katherine I O'RourkeAmir N Hamir
Jan 10, 2008·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Delwyn P KeanePhilip N Bochsler
Mar 1, 2008·Nature Methods·Ryuichiro AtarashiByron Caughey
Apr 3, 2008·Veterinary Research·Christina J Sigurdson
Sep 9, 2008·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Delwyn P KeaneMichael D Samuel
Dec 25, 2008·PloS One·Michael W MillerPaul M Lukacs
Jan 14, 2009·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Terry R SprakerKatherine I O'Rourke
Jan 23, 2009·Journal of Virology·Cristiano CoronaCristina Casalone
Jun 19, 2009·The Journal of Applied Ecology·Gideon WasserbergMichael D Samuel
Nov 11, 2009·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Terry R SprakerAru Balachandran
Jan 22, 2010·Journal of Wildlife Diseases·Daniel P Walsh, Michael W Miller
Apr 27, 2010·PLoS Pathogens·Richard A BessenJames A Wiley
Jul 23, 2011·Prion·Ryuichiro AtarashiNoriyuki Nishida
Sep 16, 2011·The Journal of General Virology·Matthew R PerrottEdward A Hoover
Dec 2, 2011·Journal of Virology·Richard A BessenJames A Wiley

❮ Previous
Next ❯

Citations

May 26, 2016·Viruses·Christina D OrrúByron Caughey
Sep 30, 2016·PLoS Pathogens·Andrew G HughsonByron Caughey
Mar 3, 2017·Journal of Virology·Clare E HooverEdward A Hoover
Oct 6, 2020·Frontiers in Bioengineering and Biotechnology·Jose A Del Rio, Isidre Ferrer
Feb 9, 2017·Annals of Clinical and Translational Neurology·Bradley R GrovemanByron Caughey
Jan 17, 2018·EFSA Journal·UNKNOWN EFSA Panel on Biological Hazards (BIOHAZ)Marion Simmons
Jun 10, 2017·BioMed Research International·Hae-Eun KangChongsuk Ryou
Jan 28, 2022·Cell and Tissue Research·Ryuichiro Atarashi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bovine Spongiform Encephalopathy

Bovine spongiform encephalopathy is a neurodegenerative disease belonging to the transmissible spongiform encephalopathies, a group of diseases including sheep scrapie and human Creutzfeldt-Jakob disease. Here is the latest research.

Bovine Spongiform Encephalopathy (MDS)

Bovine spongiform encephalopathy is a neurodegenerative disease belonging to the transmissible spongiform encephalopathies, a group of diseases including sheep scrapie and human Creutzfeldt-Jakob disease. Here is the latest research.