Seeking completeness in bacterial mutant hunts

Current Opinion in Microbiology
Nina R Salama, Colin Manoil

Abstract

The identification of most or all of the genetic functions that are required for a particular biological process could be achieved through phenotypic studies of high genome-coverage mutant collections. Technologies for creating such collections, in the form of mixed populations or individually arrayed sequence-defined mutants, are now available for numerous bacterial species. The analysis of mixed mutant collections using microarray-based detection procedures appears to be particularly effective in identifying functions required for complex processes such as virulence. The phenotypic analysis of sequence-defined mutant libraries provides a virtually complete identification of nonessential genes required for processes for which suitable screens can be devised. Such libraries also serve as a source of individual mutants for examining the biological relevance of gene associations revealed by transcriptional profiling or homology.

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Citations

Jun 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·D Ewen CameronJohn J Mekalanos
Sep 18, 2012·Journal of Bacteriology·Kiara HeldColin Manoil
Mar 22, 2012·MBio·Mark EnstromColin Manoil
Nov 7, 2013·MBio·Larry A GallagherColin Manoil
Sep 11, 2009·International Journal of Biological Sciences·Xinglin ZhangQingmin Wu
Dec 13, 2006·Current Opinion in Microbiology·Laura S Burrack, Darren E Higgins
Jan 12, 2007·Proceedings of the National Academy of Sciences of the United States of America·Larry A GallagherColin Manoil

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