Segmental difference of mucosal damage along the length of a mouse small intestine in an Ussing chamber

Journal of Nutritional Science and Vitaminology
Eiko InagakiYuichi Suzuki

Abstract

The Ussing chamber is often used for in vitro investigations into the transport physiology of epithelia including the small intestine. However, the morphological and functional integrity of the small intestine incubated in an Ussing chamber has been largely ignored. The present study attempted to compare the mucosal injury among different segments of the mouse small intestine when incubated in an Ussing chamber. To assess the functional damage, we measured changes in the transmural potential difference (PD) evoked by adding glucose to the mucosal solution and forskolin to the mucosal and serosal solutions. Morphological deterioration was assessed histologically. The villi in the duodenum and proximal jejunum were morphologically damaged by 2 h of incubation in an Ussing chamber and almost completely destroyed within 4 h, while crypts remained intact. The villi were moderately damaged in the distal jejunum. In contrast, the integrity of the villi and crypts was maintained in the ileum for 4 h. The basal PD and forskolin-induced PD were maintained up to 4 h of incubation in all segments. On the other hand, the glucose-induced PD was not apparent in the duodenum at 0 h, and was gradually suppressed to zero in the proximal jejunum ...Continue Reading

Citations

Oct 28, 2005·The Journal of Heredity·P R AldrichC H Michler
Jul 19, 2008·Journal of Nutritional Science and Vitaminology·Eiko Inagaki-TachibanaYuichi Suzuki
Apr 5, 2008·Journal of Nutritional Science and Vitaminology·Eiko Inagaki-TachibanaYuichi Suzuki
Nov 12, 2014·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·R Todd AlexanderHenrik Dimke
Jul 23, 2013·American Journal of Physiology. Gastrointestinal and Liver Physiology·Veronica L NovosadThomas L Clanton
Jul 28, 2013·American Journal of Physiology. Gastrointestinal and Liver Physiology·Robert W FreelMarguerite Hatch
Nov 10, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Azucena Perez-BurgosWolfgang Kunze
Sep 22, 2018·Endocrinology·Deborah A GoldspinkFiona M Gribble
Apr 13, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Atsukazu KuwaharaYoshinori Marunaka
Jun 26, 2021·Frontiers in Endocrinology·Rune E KuhreNatalia Petersen

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