PMID: 3322268Nov 1, 1987Paper

Segmental structure and protein domains in the pyruvate dehydrogenase multienzyme complex of Escherichia coli. Genetic reconstruction in vitro and 1H-n.m.r. spectroscopy

The Biochemical Journal
S E RadfordJ R Guest

Abstract

A deletion in vitro can be made in the aceEF-lpd operon encoding the pyruvate dehydrogenase multienzyme complex of Escherichia coli, which causes deletion of two of the three homologous lipoyl domains that comprise the N-terminal half of each dihydrolipoamide acetyltransferase (E2p) polypeptide chain. An active complex is still formed and 1H-n.m.r. spectroscopy of this modified complex revealed that many of the unusually sharp resonances previously attributed to conformationally mobile segments in the wild-type E2p polypeptide chains had correspondingly disappeared. A further deletion was engineered in the long (alanine + proline)-rich segment of polypeptide chain that linked the one remaining lipoyl domain to the C-terminal half of the E2p chain. 1H-n.m.r. spectroscopy of the resulting enzyme complex, which was also active, revealed a further corresponding loss in the unusually sharp resonances observed in the spectrum. These experiments strongly support the view that the sharp resonances derive, principally at least, from the three long (alanine + proline)-rich sequences which separate the three lipoyl domains and link them to the C-terminal half of the E2p chain. Closer examination of the 400 MHz 1H-n.m.r. spectra of the wil...Continue Reading

Citations

Jan 1, 1989·Annals of the New York Academy of Sciences·L J ReedD J Uhlinger
Jun 1, 1993·FEBS Letters·R S MachadoM P Williamson
Jan 1, 1989·Annals of the New York Academy of Sciences·R N Perham, L C Packman
Jan 30, 1990·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·P Dimroth
Jun 10, 2000·European Journal of Biochemistry·W R HagenK D Schnackerz
Jan 23, 2003·Protein Engineering·Richard A George, Jaap Heringa
Oct 1, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sowmini KumaranFrank Jordan
Jan 1, 1989·Annals of the New York Academy of Sciences·J R GuestG C Russell
Dec 12, 2012·Protein Science : a Publication of the Protein Society·Vishnu Priyanka Reddy ChichiliJ Sivaraman
Jun 27, 2009·Angewandte Chemie·Geoffrey W PlattSheena E Radford
Aug 7, 2020·Biophysical Journal·Ivana GaziovaAndres F Oberhauser
Mar 1, 1990·Research in Microbiology·P Dimroth
Mar 31, 2015·Biomacromolecules·Andrew J BaldwinPeter R Ellis
Feb 6, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Urartu Ozgur Safak SekerHilmi Volkan Demir

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.