Segmented flow sampling with push-pull theta pipettes

The Analyst
Anumita Saha-ShahLane A Baker

Abstract

We report development of a mobile and easy-to-fabricate theta pipette microfluidic device for segmented flow sampling. The theta pipettes were also used as electrospray emitters for analysis of sub-nanoliter segments, which resulted in delivery of analyte to the vacuum inlet of the mass spectrometer without multiple transfer steps. Theta pipette probes enable sample collection with high spatial resolution due to micron or smaller sized probe inlets and can be used to manipulate aqueous segments in the range of 200 pL to tens of nanoliters. Optimized conditions can enable sampling with high spatial and temporal resolution, suitable for chemical monitoring in biological samples and studies of sample heterogeneity. Intercellular heterogeneity among Allium cepa cells was studied by collecting cytoplasm from multiple cells using a single probe. Extracted cytoplasm was analyzed in a fast and high throughput manner by direct electrospray mass spectrometry of segmented sample from the probe tip.

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Citations

Apr 1, 2017·The Analyst·Anumita Saha-ShahLane A Baker
Apr 29, 2018·Analytical and Bioanalytical Chemistry·Elizabeth M Yuill, Lane A Baker
Nov 15, 2018·The Analyst·Kyle D DuncanIngela Lanekoff
Oct 25, 2017·Analytical Methods : Advancing Methods and Applications·Rosemary M OnjikoPeter Nemes
Feb 1, 2019·Current Opinion in Electrochemistry·Lane A Baker, Gargi S Jagdale
Jun 7, 2018·Analytical Chemistry·Ruichuan YinJulia Laskin
Jan 21, 2017·Analytical Chemistry·Mallikarjunarao GanesanaB Jill Venton
Jan 21, 2017·Analytical Chemistry·Wenqing ShiLane A Baker
Jun 14, 2019·Analytical Chemistry·Anumita Saha-ShahBenjamin A Garcia
Apr 30, 2019·Analytical Chemistry·Daniel J Steyer, Robert T Kennedy

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