Segmented GFP-like aptamer probes for functional imaging of viral genome trafficking

Virus Research
Irina B TsvetkovaBogdan Dragnea

Abstract

Recently developed GFP-like RNA aptamers harbor a few unique potential benefits for in vivo RNA imaging applications, including co-packaging of viral genomes. Here we examine them in the context of co-packaging of RNA strands during virion assembly and trafficking. The approach is applicable both in vitro and in vivo, thus bridging an existing methodological gap. We have found that splitting the aptamer sequence in the loop region into two separate parts allows for subsequent self-assembly into a functional unit, which preserves the dye-binding pocket. In presence of the dye, virus-like particles encapsulating segmented GFP-like aptamers provided bright fluorescence emission and showed negligible bleaching due to continuous chromophore exchange: two desirable characteristics for real-time in vivo single particle studies requiring a broader dynamic range than currently available. Proof-of-principle in vivo imaging experiments confirmed detectability of aptamer-loaded virus-like particles in barley root cells even in presence of significant autofluorescence background.

References

Nov 11, 1998·Molecular Cell·E BertrandR M Long
Oct 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Diana P BratuSanjay Tyagi
Dec 4, 2003·Journal of the American Chemical Society·Jeremy R BabendureRoger Y Tsien
Sep 19, 2006·Virology·Sonia ZúñigaLuis Enjuanes
Dec 4, 2009·The Journal of General Virology·Edward C HutchinsonPaul Digard
Mar 1, 2000·Molecular Plant Pathology·C C Kao, K Sivakumaran
Jul 29, 2010·Physiological Reviews·Dmitriy M ChudakovKonstantin A Lukyanov
Jul 30, 2011·Science·Jeremy S PaigeSamie R Jaffrey
Mar 10, 2012·Science·Jeremy S PaigeSamie R Jaffrey
Nov 5, 2013·The Analyst·Tucker J Carrocci, Aaron A Hoskins
Jan 8, 2014·Journal of the American Chemical Society·Wenjiao SongSamie R Jaffrey
Jun 17, 2014·ACS Synthetic Biology·Tucker A RogersWade W Grabow
Oct 23, 2014·Journal of the American Chemical Society·Grigory S FilonovSamie R Jaffrey

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Citations

Jul 6, 2016·Frontiers in Chemistry·Franziska Pfeiffer, Günter Mayer
Jan 4, 2018·International Journal of Molecular Sciences·Farah BouheddaMichael Ryckelynck
Jun 16, 2016·Chembiochem : a European Journal of Chemical Biology·Nanami Kikuchi, Dmitry M Kolpashchikov
Feb 18, 2021·ChemMedChem·Pedro GamaRafael Vazquez-Duhalt

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