Segmented molecular design of self-healing proteinaceous materials

Scientific Reports
Veikko SariolaMelik C Demirel

Abstract

Hierarchical assembly of self-healing adhesive proteins creates strong and robust structural and interfacial materials, but understanding of the molecular design and structure-property relationships of structural proteins remains unclear. Elucidating this relationship would allow rational design of next generation genetically engineered self-healing structural proteins. Here we report a general self-healing and -assembly strategy based on a multiphase recombinant protein based material. Segmented structure of the protein shows soft glycine- and tyrosine-rich segments with self-healing capability and hard beta-sheet segments. The soft segments are strongly plasticized by water, lowering the self-healing temperature close to body temperature. The hard segments self-assemble into nanoconfined domains to reinforce the material. The healing strength scales sublinearly with contact time, which associates with diffusion and wetting of autohesion. The finding suggests that recombinant structural proteins from heterologous expression have potential as strong and repairable engineering materials.

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Citations

May 26, 2016·Proceedings of the National Academy of Sciences of the United States of America·Huihun JungMelik C Demirel
Jul 16, 2016·ACS Applied Materials & Interfaces·David GaddesMelik C Demirel
Sep 27, 2018·Plastic and Reconstructive Surgery. Global Open·Ashley N LeberfingerDino J Ravnic
Mar 9, 2019·Frontiers in Chemistry·Abdon Pena-Francesch, Melik C Demirel
Jul 29, 2020·Nature Materials·Abdon Pena-FranceschMetin Sitti
Jan 22, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Wonkyung AhnEun Jung Lee
Feb 18, 2021·The Journal of Physical Chemistry. B·Romeo C A DubiniPetra Rovó
Mar 12, 2018·ACS Biomaterials Science & Engineering·Abdon Pena-FranceschMelik C Demirel
Apr 19, 2019·ACS Applied Materials & Interfaces·Dian YuanIca Manas-Zloczower
Nov 18, 2020·ACS Synthetic Biology·Jasmine M HersheweMichael C Jewett

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Methods Mentioned

BETA
differential scanning calorimetry
X-ray
infrared spectroscopy
co-crystallization
NMR

Software Mentioned

OriginPro
hpdec
MDI
OMNIC
TopSpin
Jade X
av
Gromacs simulation engine

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