Segmenting clustered nuclei using H-minima transform-based marker extraction and contour parameterization

IEEE Transactions on Bio-medical Engineering
Chanho Jung, Changick Kim

Abstract

In this letter, we present a novel watershed-based method for segmentation of cervical and breast cell images. We formulate the segmentation of clustered nuclei as an optimization problem. A hypothesis concerning the nuclei, which involves a priori knowledge with respect to the shape of nuclei, is tested to solve the optimization problem. We first apply the distance transform to the clustered nuclei. A marker extraction scheme based on the H-minima transform is introduced to obtain the optimal segmentation result from the distance map. In order to estimate the optimal h-value, a size-invariant segmentation distortion evaluation function is defined based on the fitting residuals between the segmented region boundaries and fitted models. Ellipsoidal modeling of contours is introduced to adjust nuclei contours for more effective analysis. Experiments on a variety of real microscopic cell images show that the proposed method yields more accurate segmentation results than the state-of-the-art watershed-based methods.

References

Mar 24, 1998·IEEE Transactions on Bio-medical Engineering·H S WuJ Gil
Sep 20, 2005·IEEE Transactions on Information Technology in Biomedicine : a Publication of the IEEE Engineering in Medicine and Biology Society·Lin YangDavid J Foran
Sep 30, 2005·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·Francesco RaimondoIoannis Kostopoulos
Nov 2, 2006·Genome Biology·Anne E CarpenterDavid M Sabatini
Oct 20, 2007·Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society·Metin N GurcanJoel Saltz
Jan 24, 2009·IEEE Transactions on Medical Imaging·Hela MasmoudiMarios A Gavrielides
Mar 11, 2009·IEEE Transactions on Information Technology in Biomedicine : a Publication of the IEEE Engineering in Medicine and Biology Society·Xiaobo ZhouStephen T C Wong
Mar 11, 2009·IEEE Transactions on Bio-medical Engineering·Jierong Cheng, Jagath C Rajapakse

❮ Previous
Next ❯

Citations

Nov 16, 2012·PloS One·Can Fahrettin KoyuncuCigdem Gunduz-Demir
Jan 1, 2011·Journal of Pathology Informatics·Andreas KårsnäsRasmus Larsen
Jan 15, 2015·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society· Zhi LuAndrew P Bradley
Mar 8, 2016·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Can Fahrettin KoyuncuCigdem Gunduz-Demir
Nov 26, 2015·Medical Image Analysis·Xiaofan ZhangShaoting Zhang
Apr 10, 2013·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Cheng ChenGustavo K Rohde
Mar 29, 2014·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Chanho Jung, Changick Kim
Jun 1, 2014·IEEE Transactions on Bio-medical Engineering·Ji GeYu Sun
Sep 6, 2014·IEEE Journal of Biomedical and Health Informatics·Hongming XuMrinal Mandal
Feb 22, 2014·IEEE Transactions on Bio-medical Engineering·Fuyong XingLin Yang
Jul 1, 2014·IEEE/ACM Transactions on Computational Biology and Bioinformatics·Hai SuLin Yang
Jul 24, 2014·IEEE Journal of Biomedical and Health Informatics·Wojciech TarnawskiAldo Ferrari
Jul 4, 2012·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·Marina E Plissiti, Christophoros Nikou
Oct 30, 2015·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·Sahar ZafariHeikki Haario
Feb 26, 2016·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Mohammed Al-MamunRosemary Bass
Apr 14, 2015·Journal of Microscopy·Chao ZhangTuan D Pham
Dec 8, 2015·IEEE Journal of Biomedical and Health Informatics·Jie SongZhichao Lian
Mar 30, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jia-Mei ChenJuan Liu
Sep 14, 2018·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Can Fahrettin KoyuncuCigdem Gunduz-Demir
Jun 28, 2017·Journal of Medical Imaging·Faliu YiGuanghua Xiao
Oct 4, 2016·Scientific Reports·Cheng LuAnant Madabhushi
Aug 30, 2017·Neuroinformatics·Karolina NurzynskaAdam Piorkowski
Mar 14, 2013·Optics Express·Ahmed El Mallahi, Frank Dubois
Oct 5, 2018·BMC Bioinformatics·Yousef Al-KofahiMirabela Rusu
Sep 25, 2017·Neuroinformatics·Tianyu HuQian Liu
Oct 18, 2019·International Journal of Molecular Sciences·Teresa ConceiçãoMaria João M Vasconcelos
Oct 23, 2018·Journal of Healthcare Engineering·Khin Yadanar WinManasanan Raveesunthornkiat
May 22, 2020·Medical Image Analysis·Can Fahrettin KoyuncuCigdem Gunduz-Demir

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Ductal

Ductal carcinoma is a malignant neoplasm involving the ductal systems of any of a number of organs, such as the mammary glands, pancreas, prostate or lacrimal gland. Discover the latest research on ductal carcinoma here.

Related Papers

IEEE Transactions on Bio-medical Engineering
Jierong Cheng, Jagath C Rajapakse
IEEE Transactions on Bio-medical Engineering
Chanho JungSukjoong Oh
Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Joakim LindbladAlla Zaltsman
Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Gang LinBadrinath Roysam
© 2021 Meta ULC. All rights reserved