Segregating metabolic processes into different microbial cells accelerates the consumption of inhibitory substrates

The ISME Journal
Elin E Lilja, David R Johnson

Abstract

Different microbial cell types typically specialize at performing different metabolic processes. A canonical example is substrate cross-feeding, where one cell type consumes a primary substrate into an intermediate and another cell type consumes the intermediate. While substrate cross-feeding is widely observed, its consequences on ecosystem processes is often unclear. How does substrate cross-feeding affect the rate or extent of substrate consumption? We hypothesized that substrate cross-feeding eliminates competition between different enzymes and reduces the accumulation of growth-inhibiting intermediates, thus accelerating substrate consumption. We tested this hypothesis using isogenic mutants of the bacterium Pseudomonas stutzeri that either completely consume nitrate to dinitrogen gas or cross-feed the intermediate nitrite. We demonstrate that nitrite cross-feeding eliminates inter-enzyme competition and, in turn, reduces nitrite accumulation. We further demonstrate that nitrite cross-feeding accelerates substrate consumption, but only when nitrite has growth-inhibiting effects. Knowledge about inter-enzyme competition and the inhibitory effects of intermediates could therefore be important for deciding how to best segrega...Continue Reading

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Citations

Apr 28, 2017·Environmental Microbiology·Matteo CavaliereJosé I Jiménez
Jun 1, 2017·Nature Communications·Christopher E LawsonDaniel R Noguera
Feb 28, 2020·FEMS Microbiology Ecology·Marian L SchmidtVincent J Denef
Feb 16, 2017·BMC Evolutionary Biology·Elin E Lilja, David R Johnson
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Oct 19, 2019·The ISME Journal·Emily J ZakemMichael J Follows
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Aug 15, 2017·Trends in Microbiology·Sara HallinChristopher M Jones

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