Segregation of Bad from lipid rafts is implicated in the induction of apoptosis

The Journal of Immunology : Official Journal of the American Association of Immunologists
Verónica AyllónA Rebollo

Abstract

Many molecules relocate subcellularly in cells undergoing apoptosis. Using coimmunoprecipitation experiments we demonstrate that Bad is not associated to 14-3-3 protein, suggesting a new mechanism for the control of the proapoptotic role of Bad. Here we show, by confocal microscopy and cellular fractionation, that Bad is attached to lipid rafts in IL-4-stimulated cells and thymocytes while associated with mitochondria in IL-4-deprived cells. Disruption of lipid rafts by methyl-beta-cyclodextrin treatment induces segregation of Bad from rafts, which correlates with apoptosis. Our results suggest that the interaction of Bad with rafts is a dynamic process regulated by IL-4 and involved in the control of apoptosis.

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Citations

Dec 10, 2002·Annual Review of Physiology·Krishnakumar Balasubramanian, Alan J Schroit
Jun 10, 2006·Apoptosis : an International Journal on Programmed Cell Death·E MalisseinD Troutaud
Nov 6, 2012·Cellular Logistics·Jean-Philippe Semblat, Christian Doerig
Nov 5, 2003·Journal of Cellular and Molecular Medicine·Alexandrina Burlacu
Sep 2, 2005·Planta·Riyaz A Bhat, Ralph Panstruga
Nov 6, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yang-Yi FanRobert S Chapkin
Jun 11, 2005·Cell Death and Differentiation·T GarofaloM Sorice
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Nov 3, 2015·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Keiichi Motoyama
Jul 2, 2003·Seminars in Arthritis and Rheumatism·Duane R Schultz, William J Harrington
Oct 31, 2003·Biochimie·Alphonse GarciaAngelita Rebollo
Oct 31, 2003·Biochimie·Alphonse GarciaAngelita Rebollo
Apr 15, 2004·Chemistry and Physics of Lipids·Ileana M Cristea, Mauro Degli Esposti
Nov 17, 2007·Genes & Development·Devanjan Sikder, Thomas Kodadek

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