PMID: 9532744Apr 9, 1998Paper

Segregation pattern of kanamycin resistance marker in Azotobacter vinelandii did not show the constraints expected in a polyploid bacterium

FEMS Microbiology Letters
L PulakatN Gavini

Abstract

It was suggested that Azotobacter vinelandii cells contain about 80 copies of their chromosome and when foreign genes are introduced into the cell, it took several generations for them to spread to all 80 chromosomes even in the presence of selection. In contrast, the fact that many recessive mutants can be isolated from A. vinelandii without the constraints expected for a cell that has 80 copies of its chromosome argued against this organism being highly polyploid. We have investigated the segregation of a kanamycin resistant genetic marker under non-selective conditions in A. vinelandii. Plasmid DNA was used to introduce the kanamycin resistance gene onto the A. vinelandii chromosome at the nifY locus by homologous recombination. The transformants were identified from non-transformants with the aid of replica plating, and hence the colonies examined for segregation of the genetic marker were never subjected to kanamycin selection. In spite of growing the transformants in the absence of selection pressure, no segregant that lacked the kanamycin resistance gene was scored. These analyses suggested that the segregation of the kanamycin marker in A. vinelandii did not exhibit any constraints expected in a highly polyploid bacterium.

References

Sep 1, 1979·Journal of Bacteriology·W J Page, M von Tigerstrom
Dec 1, 1992·Genetics·R MaldonadoJ Casadesús
Jan 1, 1990·Journal of Bacteriology·H E Kubitschek
Jun 1, 1989·Journal of Bacteriology·P NagpalH K Das
Jan 1, 1987·Annual Review of Microbiology·C Kennedy, A Toukdarian
Jan 1, 1971·Journal of Bacteriology·H L SadoffB Loperfido
Jan 1, 1968·Canadian Journal of Microbiology·G W Strandberg, P W Wilson
Jul 1, 1994·Journal of Bacteriology·R MaldonadoJ Casadesús
Jan 1, 1993·Annual Review of Microbiology·W D Donachie
Dec 1, 1993·Molecular & General Genetics : MGG·A C Manna, H K Das
Jan 26, 1996·The Journal of Biological Chemistry·L PulakatN Gavini
Sep 1, 1987·Molecular & General Genetics : MGG·A Contreras, J Casadesús

❮ Previous
Next ❯

Citations

Mar 14, 2018·Microbiology·Jesse D Noar, Jose M Bruno-Bárcena
Feb 9, 2016·Journal of Applied Microbiology·L J EberhartB M Barney

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.