PMID: 9166726Jun 1, 1997Paper

Select alterations in protein kinases and phosphatases during apoptosis of differentiated PC12 cells

Journal of Neurochemistry
P K DavisG V Johnson

Abstract

The involvement of cell cycle-regulatory proteins in apoptosis of neuronally differentiated PC12 cells induced by the removal of nerve growth factor and serum was examined. Three major findings are presented. (1) Cdc2 kinase protein levels increased fivefold in apoptotic PC12 cells by day 3 of serum and nerve growth factor deprivation. Histone H1 kinase activity was increased significantly in p13(suc1) precipitates of apoptotic PC12 cells, which was due to increased activation and/or expression of cdc2 kinase. (2) The protein levels of cyclin-dependent kinase 4, cyclin D, and proliferating cell nuclear antigen that are normally expressed in the cell cycle were increased during neuronal PC12 cell apoptosis. (3) The levels of the catalytic subunit, but not the regulatory subunit of the calcium/calmodulin-dependent protein phosphatase 2B, decreased significantly concomitant with a significant decrease in protein phosphatase 2B activity early in the apoptotic process. Protein phosphatase 2A activity decreased slightly but significantly after 3 days of serum and nerve growth factor deprivation, and no alterations in protein phosphatase 1 were observed during the apoptotic process. These data demonstrate that certain cell cycle-regul...Continue Reading

Citations

May 28, 1998·The Journal of Biological Chemistry·R P Guttmann, G V Johnson
Nov 1, 2000·Cell Motility and the Cytoskeleton·H Ksiezak-RedingD W Dickson
Jun 18, 2002·Journal of Neurochemistry·Janusz Tucholski, Gail V W Johnson
Sep 11, 2003·Journal of Hematotherapy & Stem Cell Research·Gurveen SaberwalSuneel D Mundle
Mar 11, 2004·Journal of Neurochemistry·Shirley B Shelton, Gail V W Johnson
Oct 10, 2001·Journal of Neuroscience Research·S B Shelton, G V Johnson
Sep 5, 2002·Journal of Neuroscience Research·Cristina Martín de la VegaMatilde Salinas
Jun 18, 2002·Journal of Neurochemistry·Jianwen ZhangGail V W Johnson
Oct 21, 2004·The Journal of Biological Chemistry·Jae-Hyeon Cho, Gail V W Johnson
Feb 13, 2001·Biochemical and Biophysical Research Communications·P KerokoskiT Pirttilä
Nov 27, 2001·Brain Research·P Mookherjee, G V Johnson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.