Abstract
The number of biocatalysts needed for biotechnological applications is increasing rapidly, necessitating the development of novel identification systems. To date, strategies based on high-throughput screening and selection are applied, with the latter enabling a desired phenotype to survive under appropriate conditions. The groups of Witholt and de Lorenzo have devised a novel selection system, comprising a transcriptional regulator that is activated upon binding to the product of a biocatalytic reaction, which paves the way for easier selection of many different biocatalysts.
References
Feb 24, 2001·Nature·A SchmidB Witholt
Dec 17, 2002·Proceedings of the National Academy of Sciences of the United States of America·Kathleen BakerVirginia W Cornish
May 9, 2003·Nature·Loren L LoogerHomme W Hellinga
Sep 2, 2003·Trends in Biotechnology·Ana Fernandez-GacioJacques Fastrez
Aug 7, 2004·Current Opinion in Biotechnology·Stefan BeckerHarald Kolmar
Dec 21, 2004·Nature Biotechnology·Taku UchiyamaKazuya Watanabe
Jan 18, 2005·Nucleic Acids Research·Jae-Ho ShinZvi Kelman
Apr 29, 2005·Biomolecular Engineering·Linda G Otten, Wim J Quax
Jun 3, 2005·Nature Reviews. Microbiology·Patrick Lorenz, Jürgen Eck
Dec 13, 2005·Chembiochem : a European Journal of Chemical Biology·Melloney J DrögeWim J Quax
Dec 22, 2005·Applied Microbiology and Biotechnology·Joachim Jose
Feb 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Stephan van Sint FietBernard Witholt
Feb 16, 2006·Environmental Microbiology·William W MohnVíctor de Lorenzo
Mar 26, 2016·Current Opinion in Biotechnology·Caroline BaroukhJean-Philippe Steyer
Citations
Dec 4, 2013·ACS Synthetic Biology·Su-Lim ChoiSeung-Goo Lee
Oct 3, 2006·Applied and Environmental Microbiology·Helge HenningKarl-Erich Jaeger
May 19, 2010·International Journal of Molecular Sciences·Pedro Fernandes
Apr 26, 2011·Protein Expression and Purification·Pablo FuciñosM Luisa Rúa
May 16, 2009·Biotechnology Advances·J D CarballeiraJ V Sinisterra
Apr 24, 2007·The FEBS Journal·Ykelien L BoersmaWim J Quax
Apr 13, 2013·The FEBS Journal·Teunke van RossumJohn van der Oost
Jun 2, 2012·The Quarterly Journal of Experimental Psychology : QJEP·Ángel TabulloAlberto Yorio