Selected compounds derived from Moutan Cortex stimulated glucose uptake and glycogen synthesis via AMPK activation in human HepG2 cells

Journal of Ethnopharmacology
Do Thi HaKihwan Bae

Abstract

To evaluate the effect of selected compounds derived from Moutan Cortex on glucose uptake and glycogen synthesis associated with AMPK activation in insulin-resistant human HepG2 cell. The effect of isolated compounds (1-16) on glucose uptake and glycogen synthesis was performed using HepG2 cells. The western blot was used to determine the expression of AMPK and its downstream substrates, ACC, p-ACC, and p-GSK-3beta. The effects of the 16 compounds from Moutan Cortex on glucose metabolism in HepG2 cells under high glucose conditions were evaluated. Compounds 2, 3, and 6 displayed highly potent effects on the stimulation of glucose uptake and glycogen synthesis in human HepG2 cells under high glucose conditions. Compounds 2, 3, and 6 phosphorylate AMPK (AMP-activated protein kinase), and resulted in increased phosphorylation of GSK-3beta and suppression of lipogenic expression (ACC and FAS) in a dose-dependent manner. Compounds 2, 3, and 6 also demonstrated interesting, strong eNOS phosphorylation in human umbilical vein endothelial cells (HUVECs). Compounds 1, 4, 5-12, and 14 displayed considerable effects on hepatic glucose production, AMPK activation, and phosphorylation of GSK-3beta in HepG2 cells under high glucose condition...Continue Reading

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Citations

May 31, 2012·Archives of Pharmacal Research·Yun-Hyeok ChoiJung Ho Choi
Mar 31, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Hai-Ming ZhangRui Chen
Jun 8, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhiqiang WangPeigen Xiao
Dec 22, 2017·International Journal of Molecular Sciences·Solomon Habtemariam
Feb 14, 2021·International Journal of Biological Macromolecules·Yuanpei LianLiang Feng
Jul 3, 2021·International Journal of Molecular Sciences·Kyung-Ran ParkHyung-Mun Yun

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