PMID: 9435584Jan 22, 1998Paper

Selected isozymes of PKC contribute to augmented growth of fetal and neonatal bovine PA adventitial fibroblasts

The American Journal of Physiology
Mita DasE C Dempsey

Abstract

We sought to determine which isozymes of protein kinase C (PKC) contribute to the increased proliferation of immature bovine pulmonary artery (PA) adventitial fibroblasts. Seven were identified in lysates of neonatal PA fibroblasts by Western blot: three Ca2+ dependent (alpha, beta I, and beta II) and four Ca2+ independent (delta, epsilon, zeta, and mu). Four isozymes (gamma, eta, theta, and iota) were not detected in fibroblasts isolated at any developmental stage. Of the seven detected isozymes, only PKC-alpha and -beta II protein levels were higher in fetal and neonatal cells compared with adult fibroblasts. Their role in the enhanced growth of immature fibroblasts was then evaluated. The isozyme nonselective PKC inhibitor Ro-31-8220 was first compared with GF-109203X, a structural analog of Ro-31-8220 with relative specificity for the Ca(2+)-dependent isozymes of PKC. GF-109203X selectively inhibited the growth of immature cells and was nearly as potent as Ro-31-8220. Go-6976, a more specific inhibitor of the Ca(2+)-dependent isozymes, mimicked the antiproliferative effect of GF-109203X. PKC downregulation with 1 microM phorbol 12-myristate 13-acetate had the same selective antiproliferative effect on immature fibroblasts a...Continue Reading

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Citations

Aug 25, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·E C DempseyR O Messing
Sep 15, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kurt R StenmarkIvan F McMurtry
Apr 12, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·M DasK R Stenmark
Jun 8, 2001·Critical Care Medicine·J W BerkenboschT Perreault

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