Selected non-ionic biological detergents enhance signal intensity of intact bovine serum albumin by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry

European Journal of Mass Spectrometry
Craig S Brinkworth, David J Bourne

Abstract

Recently, we showed that the signal intensity of intact protein by matrix-assisted laser desorption/ionisation (MALDI) mass spectro-metry measurement can be enhanced at least an order of magnitude by the addition of Tween80 to the analyte solution. We did not ascertain whether this effect was limited to Tween80 or if it was more universal of biological detergents. This paper discusses our investigations into this question. A variety of chemically diverse detergents were added to analyte solutions containing bovine serum albumin (BSA) to determine whether there was significant signal enhancement. The addition of Tween20, Tween80, Triton X100 and Triton X-114 improved the attainable sensitivity of intact protein MALDI mass spectrometry compared to spectra acquired without detergent. In some cases there was considerable improvement in signal--for example, with Triton X-100 two charge states (the +1 and +2) of BSA (3.9 fmol) could easily be observed. Another advantage of this process is that the detergent can be added directly to the matrix solution reducing sample handling and preparation time. We propose this phenomenon results from the ability of these detergents to increase the solubility of the protein via hydrophobic and hydr...Continue Reading

References

Nov 14, 1997·Analytical Chemistry·C Fenselau
Jan 13, 2006·Methods in Enzymology·Robert J ChalkleyMichael A Baldwin
Jan 18, 2006·Methods in Enzymology·Lan Huang, A L Burlingame
Feb 9, 2006·Combinatorial Chemistry & High Throughput Screening·Martin KussmannLaurent B Fay
Oct 19, 2006·Journal of the American Society for Mass Spectrometry·Craig S Brinkworth, David J Bourne

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