Selected Reaction Monitoring (SRM) Analysis of Epidermal Growth Factor Receptor (EGFR) in Formalin Fixed Tumor Tissue.

Clinical Proteomics
Todd HembroughJon Burrows

Abstract

Analysis of key therapeutic targets such as epidermal growth factor receptor (EGFR) in clinical tissue samples is typically done by immunohistochemistry (IHC) and is only subjectively quantitative through a narrow dynamic range. The development of a standardized, highly-sensitive, linear, and quantitative assay for EGFR for use in patient tumor tissue carries high potential for identifying those patients most likely to benefit from EGFR-targeted therapies. A mass spectrometry-based Selected Reaction Monitoring (SRM) assay for the EGFR protein (EGFR-SRM) was developed utilizing the Liquid Tissue®-SRM technology platform. Tissue culture cells (n = 4) were analyzed by enzyme-linked immunosorbent assay (ELISA) to establish quantitative EGFR levels. Matching formalin fixed cultures were analyzed by the EGFR-SRM assay and benchmarked against immunoassay of the non-fixed cultured cells. Xenograft human tumor tissue (n = 10) of non-small cell lung cancer (NSCLC) origin and NSCLC patient tumor tissue samples (n = 23) were microdissected and the EGFR-SRM assay performed on Liquid Tissue lysates prepared from microdissected tissue. Quantitative curves and linear regression curves for correlation between immunoassay and SRM methodology wer...Continue Reading

References

Mar 1, 1992·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·C R Taylor
Feb 1, 1991·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J T Mason, T J O'Leary
Apr 29, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K IkedaM Monden
Apr 5, 2001·The New England Journal of Medicine·B J DrukerC L Sawyers
Oct 13, 2001·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Y Yarden
Mar 14, 2003·Nature·Ruedi Aebersold, Matthias Mann
Feb 10, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·Vladimir K RaitJeffrey T Mason
Feb 18, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·Vladimir K RaitJeffrey T Mason
Aug 11, 2005·Molecular & Cellular Proteomics : MCP·Brian L HoodDavid B Krizman
Jan 13, 2006·Nature Chemical Biology·Shao-En Ong, Matthias Mann
Mar 15, 2006·BioTechniques·DaRue A PrietoDavid B Krizman
Mar 15, 2006·Journal of Experimental Therapeutics & Oncology·John Fruehauf
Jun 9, 2006·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·C R Taylor
Jul 13, 2006·Archives of Pathology & Laboratory Medicine·Melissa CreggerDavid L Rimm
May 2, 2007·Advances in Anatomic Pathology·Trishe Y-M Leong, Anthony S-Y Leong
May 29, 2007·Laboratory Investigation; a Journal of Technical Methods and Pathology·Carol B FowlerJeffrey T Mason
Feb 19, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Vyomesh PatelJ Silvio Gutkind
Oct 14, 2008·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Wang CheungAnirban Maitra
Oct 20, 2009·Molecular & Cellular Proteomics : MCP·Paul A RudnickStephen E Stein
Nov 4, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Humberto Lara-GuerraNatasha B Leighl
Dec 29, 2009·Journal of Pharmacological and Toxicological Methods·Noriko DaneshtalabJohn S Smeda
Mar 25, 2010·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Valsamo K AnagnostouDavid L Rimm
Aug 27, 2010·Current Opinion in Oncology·Igor Vivanco, Ingo K Mellinghoff
Sep 2, 2010·Nature Methods·Tommy NilssonJohn J M Bergeron
Oct 14, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Celine MascauxFred R Hirsch

❮ Previous
Next ❯

Citations

Oct 31, 2014·Nature Methods·Javier A AlfaroPaul C Boutros
Jul 7, 2015·Expert Review of Proteomics·Nai Chien YeatJimmy Lin
Feb 27, 2015·Expert Review of Proteomics·Petra HudlerRadovan Komel
Jul 28, 2015·Expert Review of Molecular Diagnostics·Stefanie Boellner, Karl-Friedrich Becker
Jan 24, 2013·Proteomics. Clinical Applications·Kenneth D ColeLili Wang
Oct 17, 2013·Journal of Mass Spectrometry : JMS·Jason D TheisAhmet Dogan
Nov 20, 2015·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Daniel V T CatenacciTodd Hembrough
Apr 19, 2015·Breast Cancer Research : BCR·Paolo NuciforoMaurizio Scaltriti
Nov 29, 2014·Journal of Clinical Pathology·David KrizmanRobert Parry
Oct 1, 2016·Annals of Oncology : Official Journal of the European Society for Medical Oncology·E AnY-J Bang
Jan 17, 2017·Laboratory Investigation; a Journal of Technical Methods and Pathology·Maria I TokiDavid L Rimm
Jan 30, 2019·International Journal of Cancer. Journal International Du Cancer·Ofra NovoplanskyMoshe Elkabets
Mar 5, 2014·Applied Immunohistochemistry & Molecular Morphology : AIMM·Mark A ApplebaumSteven G DuBois
Aug 7, 2017·Biomedit︠s︡inskai︠a︡ khimii︠a︡·S E NovikovaA I Archakov
Jul 11, 2019·CNS Oncology·Samia AsifShahzad Raza
Dec 12, 2019·Journal of Neuro-oncology·Lauren R SchaffAndrew L Lin
Oct 5, 2017·Proteomics. Clinical Applications·Coşkun GüzelTheo M Luider
Jul 21, 2017·NPJ Systems Biology and Applications·Derek Croote, Stephen R Quake
May 31, 2017·International Journal of Molecular Sciences·Jia-Lin YangPhilip J Crowe
Nov 23, 2018·Cancer Discovery·Francisco Sanchez-VegaYelena Y Janjigian
Jan 1, 2017·Annals of Oncology : Official Journal of the European Society for Medical Oncology·E AnY-J Bang

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
xenograft
surgical resection
dissection
enzyme-linked immunosorbent assay
glycosylation
sandwich immunoassay
protein assay
for

Software Mentioned

BLAST
Pinpoint1
Xcalibur2
Excel

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.