Selected Th1 and Th2 cytokine mRNA expression by CD4(+) T cells isolated from inflamed human gingival tissues

Clinical and Experimental Immunology
K FujihashiH Kiyono

Abstract

Elevated numbers of plasma cells are associated with localized and chronically inflamed gingiva of patients with adult periodontitis. However, only limited information is currently available as to how cytokines produced by CD4(+) T cells are involved in these increased B cell responses in affected gingival tissues. When gingival mononuclear cells (GMC) were isolated from inflamed tissues and examined by flow cytometry, approximately 20-30% of lymphocytes were CD4(+) T cells. For the analysis of Th1 and Th2 cytokine expression by these CD4(+) T cells, RNA was extracted and reverse transcriptase-polymerase chain reaction (RT-PCR) was performed by using specific 5' and 3' primers for interferon-gamma (IFN-gamma) and IL-2 (Th1), IL-4, IL-5, IL-6, IL-10 and IL-13 (Th2) and beta-actin (internal control). Two distinct cytokine profiles were noted based on the expression of selected Th1 and Th2 cytokines, where one pattern was represented by expression of mRNA for IFN-gamma, IL-6, IL-10 and IL-13, while the second consisted of mRNA for IFN-gamma, IL-6 and IL-13. In most samples, mRNA for IL-2, IL-4 and IL-5 were not detected by cytokine-specific RT-PCR. When RNA was isolated from CD4(+) T cells of concanavalin A-stimulated peripheral b...Continue Reading

References

Jan 1, 1977·Journal of Periodontal Research·B F MacklerB M Levy
Apr 1, 1992·Journal of Periodontology·S S Socransky, A D Haffajee
Aug 1, 1991·Immunology Today·S Romagnani
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P ParronchiS Romagnani
Sep 1, 1991·The Journal of Experimental Medicine·H YsselG Peltz
Feb 8, 1990·The New England Journal of Medicine·R C Williams
May 1, 1987·Journal of Clinical Immunology·E D StoufiP P Stashenko
Nov 1, 1984·Journal of Periodontal Research·M A TaubmanD J Smith
Jan 1, 1994·Annual Review of Immunology·S Romagnani
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J PunnonenJ E de Vries
Jan 1, 1993·Annual Review of Immunology·K W MooreT R Mosmann
May 1, 1974·Journal of Periodontology·Robert J GencoSolon A Ellison

❮ Previous
Next ❯

Citations

Feb 12, 2000·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·N AlhashimiM Bakhiet
Feb 6, 2004·Journal of Clinical Periodontology·Torbjørn Breivik, Graham A W Rook
Apr 27, 2004·Periodontology 2000·Kazuhisa Yamazaki, Takako Nakajima
Apr 25, 2007·Journal of Clinical Periodontology·J R GonzalesJ Meyle
Sep 3, 2010·Journal of Periodontology·Patrícia Maria A LimaWalderez Ornelas Dutra
Feb 22, 2011·Journal of Dental Research·R KobayashiK Fujihashi
Mar 19, 2020·Journal of Dental Research·A C BelkinaB S Nikolajczyk
Aug 4, 2020·Journal of Leukocyte Biology·Ying ZhengWai Keung Leung
Jan 23, 1998·Journal of Dental Research·E Gemmell, G J Seymour
Sep 26, 2001·Acta Odontologica Scandinavica·P J BakerD C Roopenian
May 8, 2001·Journal of Periodontology·K TakahashiY Murayama
Aug 9, 2003·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Yen-Tung A Teng
Sep 14, 2007·Periodontology 2000·Yael Houri-HaddadLior Shapira
Feb 26, 2005·Clinical and Experimental Immunology·Y HosokawaT Matsuo
Oct 17, 2018·Journal of Cellular Biochemistry·Saeed Mohammadian HaftcheshmehMandana Sattari
Feb 19, 1998·Oral Microbiology and Immunology·K YamazakiK Hara
Jul 13, 2002·Journal of Periodontal Research·Rangsini MahanondaSathit Pichyangkul
Sep 1, 2005·Journal of Clinical Periodontology·Tord Berglundh, Mauro Donati
Jul 5, 2002·Journal of Dental Research·E GemmellG J Seymour
Aug 5, 1998·Journal of Periodontal Research·G E SalviS Offenbacher
May 10, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·M A Taubman, T Kawai
Mar 18, 2008·International Journal of Paediatric Dentistry·Turgut DemirHasan Kaya
Sep 14, 2007·Periodontology 2000·Xiaozhe HanMartin A Taubman
Apr 7, 2005·Journal of Clinical Periodontology·Keiso TakahashiShin Kitetsu
Jan 12, 2007·Periodontology 2000·Kazuyuki Noguchi, Isao Ishikawa
Aug 8, 2015·Periodontology 2000·José R Gonzales
Feb 24, 2001·Clinical and Experimental Immunology·D F LappinD F Kinane

❮ Previous
Next ❯

Related Concepts

Related Feeds

B cell Differentiation

Depending on the signal received through the B cell receptor and other receptors, B cells differentiate into follicular or marginal zone B cells. Here is the latest research pertaining to this differentiation process.