Selecting optimal feast-to-famine ratio for a new polyhydroxyalkanoate (PHA) production system fed by valerate-dominant sludge hydrolysate

Applied Microbiology and Biotechnology
Jiuxiao HaoXiujin Wang

Abstract

The feast-to-famine ratio (F/F) represents the extent of selective pressure during polyhydroxyalkanoate (PHA) culture selection. This study evaluated the effects of F/F on a new PHA production system by an enriched culture with valerate-dominant sludge hydrolysate and selected the optimal F/F. After the original F/F 1/3 was modified to 1/1, 1/2, 1/4, and 1/5, F/F did not affect their lengths of feast phase, but affected their biomass growth behaviors during the famine phase and PHA-producing abilities. The optimal F/F was 1/2, and compared with 1/3, it increased the maximal PHA content and the fraction of 3-hydroxyvalerate (3HV) and 3-hydroxy-2-methylvalerate (3H2MV) monomers, with higher productivity and better polymer properties. Although F/F 1/2 impaired the advantage of the dominant genus Delftia, it improved the PHA production rate while decreased biomass growth rate, meanwhile enhancing the utilization and conversion of valerate. These findings indicate that in contrast to previous studies using acetate-dominant substrate for PHA production, the new system fed by valerate-dominant substrate can adopt a higher F/F.

References

Aug 1, 1997·Applied and Environmental Microbiology·R G KranzM T Madigan
Jan 9, 2007·International Journal of Biological Macromolecules·Ching-Yee Loo, Kumar Sudesh
Mar 16, 2007·Bioresource Technology·Simon BengtssonThomas Welander
Oct 26, 2010·International Journal of Systematic and Evolutionary Microbiology·Yang JiangMark van Loosdrecht
Nov 5, 2016·International Journal of Biological Macromolecules·S MohapatraK G Achary
Dec 17, 2016·Applied Biochemistry and Biotechnology·Bo WuMing-Xiong He

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BETA
SRP043519

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BETA
NMR

Software Mentioned

Mothur

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