Selection against spermatozoa with fragmented DNA after postovulatory mating depends on the type of damage.

Reproductive Biology and Endocrinology : RB&E
Juan D HourcadeAlfonso Gutiérrez-Adán

Abstract

Before ovulation, sperm-oviduct interaction mechanisms may act as checkpoint for the selection of fertilizing spermatozoa in mammals. Postovulatory mating does not allow the sperm to attach to the oviduct, and spermatozoa may only undergo some selection processes during the transport through the female reproductive tract and/or during the zona pellucida (ZP) binding/penetration. We have induced DNA damage in spermatozoa by two treatments, (a) a scrotal heat treatment (42 degrees C, 30 min) and (b) irradiation with 137Cs gamma-rays (4 Gy, 1.25 Gy/min). The effects of the treatments were analyzed 21-25 days post heat stress or gamma-radiation. Postovulatory females mated either with treated or control males were sacrificed at Day 14 of pregnancy, and numbers of fetuses and resorptions were recorded. Both treatments decreased significantly implantation rates however, the proportion of fetuses/resorptions was only reduced in those females mated to males exposed to radiation, indicating a selection favoring fertilization of sperm with unfragmented DNA on the heat treatment group. To determine if DNA integrity is one of the keys of spermatozoa selection after postovulatory mating, we analyzed sperm DNA fragmentation by COMET assay in...Continue Reading

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Citations

Oct 22, 2013·Journal of Assisted Reproduction and Genetics·Dayanidhi KumarSatish Kumar Adiga
Feb 8, 2011·Reproductive Biology and Endocrinology : RB&E·Miriam Pérez-CrespoAlfonso Gutierrez-Adan
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Dec 12, 2017·Biology of Reproduction·Serafín Pérez-CerezalesAlfonso Gutiérrez-Adán
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Feb 15, 2018·Scientific Reports·Serafín Pérez-CerezalesAlfonso Gutiérrez-Adán
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Jan 14, 2020·Radiation and Environmental Biophysics·Daria A SkorobagatkoVolodymyr Yu Strashnyuk
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Jan 11, 2020·Frontiers in Cell and Developmental Biology·Raúl Fernández-GonzálezAlfonso Gutierrez-Adan
Feb 5, 2021·Frontiers in Cell and Developmental Biology·Mª José Izquierdo-RicoManuel Avilés
Mar 25, 2021·American Journal of Men's Health·Zhi-Da ShiYi Qiu

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

COMET
SigmaStat
CometScore

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