PMID: 2118387Aug 17, 1990Paper

Selection and application of antibodies modifying the function of beta-lactamase

Biochimica Et Biophysica Acta
E Bibi, R Laskov

Abstract

Nine monoclonal antibodies directed against class A beta-lactamases were detected and selected by a novel screening procedure based on assaying the modifications in the catalytic and stability properties of beta-lactamase in solution. Unlike conventional screening, e.g., ELISA or immunoprecipitation, the present method does not depend on firm binding and thus favors detection of low affinity antibodies. Individual antibodies were found to affect the enzymatic activity in various ways including stimulation, neutralization, protection and stabilization. Class A beta-lactamases show only 20% among members of this class. In contrast, two of our monoclonal antibodies cross-reacted with different beta-lactamases and thus demonstrate the presence of shared structural epitopes in this class of enzymes. One of the cross-reacting antibodies was elicited by sequential immunization with two different beta-lactamases. Taken together, our findings stress the importance of the screening method in antibody selection and illustrate the use of 'functional' monoclonal antibodies in the study of the structure-function relationship in an enzyme.

References

May 1, 1979·Biotechnology and Bioengineering·Y Klemes, N Citri
Apr 1, 1979·International Journal of Peptide and Protein Research·P C Moews, J R Knox
Jan 1, 1979·Annual Review of Genetics·A J Clark, G J Warren
Apr 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·N CitriN Zyk
Nov 1, 1987·Antimicrobial Agents and Chemotherapy·C J MorinR Letarte
Feb 1, 1985·European Journal of Biochemistry·K Murakami, T Yoshida
Dec 31, 1971·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·M R Pollock
May 16, 1980·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R P Ambler
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·E A NiggS J Singer
Oct 15, 1984·European Journal of Biochemistry·N CitriN Zyk
Oct 1, 1984·FEBS Letters·M Bunster, H Cid
Nov 1, 1983·Analytical Biochemistry·R C Mierendorf, R L Dimond
Sep 4, 1999·American Journal of Public Health·R E GlasgowS M Boles
Aug 31, 2013·Diabetes Care·Jesper S SørensenUNKNOWN Danish Society for Diabetes in Childhood and Adolescence

❮ Previous
Next ❯

Citations

Jul 3, 1998·The EMBO Journal·M LauwereysS Muyldermans
May 28, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Vladimir I Muronetz, Timo Korpela

❮ Previous
Next ❯

Related Concepts

Related Feeds