Selection and validation of reference genes for quantitative real-time PCR studies during Saccharomyces cerevisiae alcoholic fermentation in the presence of sulfite

International Journal of Food Microbiology
Chiara NadaiViviana Corich

Abstract

Sulfur dioxide is extensively used during industrial fermentations and contributes to determine the harsh conditions of winemaking together with low pH, high sugar content and increasing ethanol concentration. Therefore the presence of sulfite has to be considered in yeast gene expression studies to properly understand yeast behavior in technological environments such as winemaking. A reliable expression pattern can be obtained only using an appropriate reference gene set that is constitutively expressed regardless of perturbations linked to the experimental conditions. In this work we tested 15 candidate reference genes suitable for analysis of gene expression during must fermentation in the presence of sulfite. New reference genes were selected from a genome-wide expression experiment, obtained by RNA sequencing of four Saccharomyces cerevisiae wine strains grown in enological conditions. Their performance was compared to that of the most common genes used in previous studies. The most popular software based on different statistical approaches (geNorm, NormFinder and BestKeeper) were chosen to evaluate expression stability of the candidate reference genes. Validation was obtained using other wine strains by comparing normaliz...Continue Reading

References

Dec 12, 2001·Molecular and Cellular Probes·S SelveyL R Griffiths
Jan 7, 2003·Applied and Environmental Microbiology·Eric RosenfeldJean-Michel Salmon
Dec 9, 2008·International Journal of Food Microbiology·Enrico VaudanoEmilia Garcia-Moruno
Jun 7, 2012·Applied Microbiology and Biotechnology·Benoit DivolEdward Duckitt

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Citations

Nov 18, 2020·FEMS Yeast Research·Monika BorkowskaEwelina Celińska
Dec 9, 2020·Yeast·Munkhtsetseg TsedneeToru Fujiwara

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