Selection and Validation of Reference Genes for RT-qPCR normalization in different tissues of milk thistle (Silybum marianum, Gaert.)

Gene
Fulvio FlaviaParis Roberta

Abstract

Quantitative reverse transcription PCR is a sensitive technique for evaluating transcriptional profiles in different experimental datasets. To obtain a reliable quantification of the transcripts level, data normalization with stable reference genes is required. Stable reference genes are identified after analysis of their transcripts profile in every new experiment and species of interest. In Silybum marianum, a widely cultivated officinal plant, only few gene expression studies exist, and reference genes for RT-qPCR studies in the diverse plant tissues have never been investigated before. In this work, the expression stability of 10 candidate reference genes was evaluated in leaves, roots, stems and fruits of S. marianum grown under physiological environmental condition. The stability values for each candidate reference gene were calculated by four canonical statistical algorithms GeNorm, NormFinder, Bestkeeper and ΔCt method in different subsets of samples, then they were ranked with RefFinder from the most to the least suitable for normalization. Best combinations of reference genes are finally proposed for different experimental data sets, including all tissues, vegetative, and reproductive tissues separately. Three target ...Continue Reading

References

Dec 18, 2003·Biotechnology Letters·Bo-Ra KimYung-Jin Chang
Jul 31, 2004·BioTechniques·Keertan DhedaAlimuddin Zumla
Mar 23, 2005·BMC Bioinformatics·Alexey LarionovWilliam Miller
Aug 24, 2006·Genome Biology·Victor SolovyevDenis Vorobyev
May 9, 2007·Nucleic Acids Research·Andreas UntergasserJack A M Leunissen
Dec 24, 2008·BMC Plant Biology·Marino Expósito-RodríguezJosé A Pérez
Feb 24, 2009·BMC Molecular Biology·Anna R PaolacciMario Ciaffi
Jan 6, 2010·Expert Opinion on Investigational Drugs·Lei LiDalin He
Jul 7, 2010·Cell·Graziano MartelloStefano Piccolo
Sep 13, 2011·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·T T H DaoR Verpoorte
Feb 1, 2012·Plant Molecular Biology·Fuliang XieBaohong Zhang
Mar 26, 2013·BMC Plant Biology·Giulia PagliaraniW Eric van de Weg
Nov 28, 2013·Annual Review of Genetics·Donald L CourtXinhua Ji
Jun 17, 2015·Scientific Reports·Rafidah LaniKeivan Zandi
Oct 5, 2015·Plant Physiology and Biochemistry : PPB·Run-Ze SunJun Wang
Feb 26, 2016·Journal of Plant Physiology·María Torres, Purificación Corchete
Oct 21, 2016·Plant Physiology and Biochemistry : PPB·Hoda A S El-GarhyFadia El Sherif
Sep 21, 2016·International Journal of Molecular Sciences·Lauralie Mangeot-PeterGea Guerriero
Oct 11, 2017·The Plant Journal : for Cell and Molecular Biology·Yongkun LvJian Chen
Jun 11, 2019·Frontiers in Plant Science·Oren ShelefShimon Rachmilevitch

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