Selection based on morphological features of porcine embryos produced by in vitro fertilization: Timing of early cleavages and the effect of polyspermy.

Animal Science Journal = Nihon Chikusan Gakkaihō
Hiep Thi NguyenKazuhiro Kikuchi

Abstract

The aim of this study was to examine whether a morphological approach is efficient for selecting high-quality porcine embryos produced by in vitro fertilization (IVF) under high polyspermy conditions. Frozen-thawed Meishan epididymal spermatozoa showing moderate and high polyspermy were subjected to IVF (1 × 105  sperms/ml). Under conditions of moderate polyspermy, 4-cell embryos selected at 48 hr after IVF (single selection) and 8-cell embryos selected at 79 hr after IVF from the collected 4-cell embryos (double selection) showed high developmental competence. Likewise, 4- and 8-cell embryos produced by IVF under high polyspermy conditions also showed high competence for development to blastocysts. However, blastocysts derived from high polyspermy conditions had significantly fewer cells than those produced under moderate polyspermy conditions. Furthermore, the frequency of nuclear and chromosomal abnormalities in 4- and 8-cell embryos produced under conditions of high polyspermy was significantly (p < .05) higher in comparison to moderate polyspermy conditions. These findings suggest that although high polyspermy affects the frequency of nuclear and chromosomal anomalies in porcine IVF embryos, subsequent selection based on m...Continue Reading

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Citations

Jun 25, 2020·Animal Science Journal = Nihon Chikusan Gakkaihō·Hiep Thi NguyenKazuhiro Kikuchi
Oct 27, 2021·Animal Science Journal = Nihon Chikusan Gakkaihō·Hiep Thi NguyenKazuhiro Kikuchi

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Methods Mentioned

BETA
motility after collection

Software Mentioned

SE
Stata

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