Selection by drug resistance proteins located in the mitochondria of mammalian cells.

Mitochondrion
Young Geol Yoon, Michael D Koob

Abstract

Transformation of mitochondria in mammalian cells is now a technical challenge. In this report, we demonstrate that the standard drug resistant genes encoding neomycin and hygromycin phosphotransferases can potentially be used as selectable markers for mammalian mitochondrial transformation. We re-engineered the drug resistance genes to express proteins targeted to the mitochondrial matrix and confirmed the location of the proteins in the cells by fusing them with GFP and by Western blot and mitochondrial content mixing analyses. We found that the mitochondrially targeted-drug resistance proteins confer resistance to high levels of G418 and hygromycin without affecting the viability of cells.

References

Jul 1, 1978·Acta Oto-laryngologica·D Bagger-Sjöbäck, J Wersäll
Dec 1, 1990·Hearing Research·J C de GrootJ E Veldman
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T D FoxT W McMullin
May 28, 1996·Proceedings of the National Academy of Sciences of the United States of America·D F SteeleT D Fox
Mar 5, 1999·Science·D C Wallace
Jul 3, 2003·Proceedings of the National Academy of Sciences of the United States of America·Pawel GolikJaga Lazowska
Jun 1, 1964·Acta Oto-laryngologica·A J DUVALL, J WERSAELL
Jan 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Koichi OkumuraWebster K Cavenee
Sep 15, 2005·Nucleic Acids Research·Young Geol Yoon, Michael D Koob

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Citations

May 23, 2012·Mitochondrion·Adnan Khan NiaziAndré Dietrich
May 3, 2011·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Young Geol Yoon, Michael Duane Koob
Dec 3, 2010·Trends in Microbiology·Mark J Pallen
Sep 2, 2009·Traffic·Sofie J HuybrechtsMarc Fransen
Dec 31, 2010·Anatomy & Cell Biology·Young Geol YoonYoung Hyun Yoo

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