Selection for chloroquine-sensitive Plasmodium falciparum by wild Anopheles arabiensis in Southern Zambia

Malaria Journal
Sungano MharakurwaPhilip E Thuma

Abstract

The emergence of parasite drug resistance, especially Plasmodium falciparum, persists as a major obstacle for malaria control and elimination. To develop effective public health containment strategies, a clear understanding of factors that govern the emergence and spread of resistant parasites in the field is important. The current study documents selection for chloroquine-sensitive malaria parasites by wild Anopheles arabiensis in southern Zambia. In a 2,000-sq km region, mosquitoes were collected from human sleeping rooms using pyrethrum spray catches during the 2006 malaria transmission season. After morphological examination and molecular confirmation, vector mosquitoes were dissected to separate head and thorax from the abdominal section, followed by PCR screening for P. falciparum infection. Human residents of all ages were tested for P. falciparum parasitaemia by microscopy and PCR. Plasmodium falciparum infections were genotyped at the chloroquine resistance-conferring amino acid codon 76 of the PfCRT gene, using PCR and restriction enzyme digestion. In the human population there was nearly 90% prevalence of the chloroquine-resistant PfCRT K76T mutant, with no significant differences in polymorphism among smear-positive...Continue Reading

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Citations

Feb 20, 2015·Annals of the New York Academy of Sciences·Adina Heinberg, Laura Kirkman
Feb 26, 2016·Molecular Biology and Evolution·Stanislaw J GabryszewskiDavid A Fidock
Aug 12, 2015·The American Journal of Tropical Medicine and Hygiene·Liwang CuiPhilip J Rosenthal
Oct 11, 2017·The American Journal of Tropical Medicine and Hygiene·Melissa D ConradPhilip J Rosenthal
Sep 25, 2019·The Journal of Infectious Diseases·Sujata BalasubramanianJessica T Lin
Jul 1, 2020·The Journal of Infectious Diseases·Sungano MharakurwaMatthew M Ippolito

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Methods Mentioned

BETA
PCR

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