PMID: 37155Feb 1, 1979

Selection of a dexamethasone-resistant H-4-IIE-C3 rat hepatoma tissue-culture line

In Vitro
C A BarnettR P Saneto


Exposure of H-4-IIE-C3 rat hepatoma cell cultures to the synthetic glucocorticoid, dexamethasone, results in an inhibition of cellular proliferation which is not the result of steroid-induced cytolysis. A significant decrease in both the rate of DNA synthesis and DNA content precedes a detectable effect on cell number. Continuous culture of H-IIE-C3 cells in medium containing 10(-5) M dexamethasone results in the selection of a steroid-resistant cell population that has the growth characteristics of unselected sensitive cultures and shows normal steroid induction of tryosine transaminase. Selection is a slow process requiring 24 to 36 months to obtain a phenotypically stable resistant cell line, and can be subdivided into three phases--a sensitive phase, adaptation and resistance. A comparison of the karyotypes of unselected and resistant cultures shows that the selection process enriches for a dexamethasone-resistant subpopulation.


Sep 1, 1976·In Vitro·V T Mullen, C A Barnett


Sep 1, 1976·In Vitro·V T Mullen, C A Barnett
Oct 30, 1971·Lancet·M Seabright
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J N LoebL L Yeung
Aug 1, 1974·Cell·C H Sibley, G M Tomkins
Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·K R YamamotoG M Tomkins
Jul 27, 1970·Biochemical and Biophysical Research Communications·K L Lee, F T Kenney
May 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·J D Baxter, G M Tomkins
Jun 1, 1971·Endocrinology·I C HendersonJ N Loeb

Related Concepts

Tyrosine Aminotransferase
Hepatoma, Novikoff
DNA, Neoplasm
Cell Division Phases
Drug Resistance
Rats, Laboratory

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