Selection of antigenic variants of foot-and-mouth disease virus in the absence of antibodies, as revealed by an in situ assay.

The Journal of General Virology
J DíezE Domingo

Abstract

Antigenic variants of foot-and-mouth disease virus (FMDV) of serotype C (isolate C-S8c1) were selected upon serial passage of the virus in cell culture in the absence of anti-FMDV antibodies. The variants rose from frequencies of less than 10(-2) in the initial plaque-purified FMDV C-S8c1 preparation, to 0.1 to 1 in three passaged populations. The proportion of antigenic variants was quantified using a new in situ plaque immunotest. A nitrocellulose filter is applied to the agar overlay of a FMDV plaque assay, and allows recovery of infectious virus from individual plaques. A second filter is placed directly on the cell monolayer and binds enough virus to permit colorimetric visualization of plaques by an enzyme-linked assay using monoclonal antibodies (MAbs). Either all or a fraction of plaques from passaged FMDV failed to react with MAb4G3, an antibody that recognizes an epitope located within residues 144 to 150 of capsid protein VP1. Some variants rapidly dominated the viral population, and others were maintained at low levels. RNA from unreactive viruses included mutations that resulted in amino acid substitutions at the epitope recognized by MAb 4G3. We discuss models for the selection of antigenic variants of FMDV in the...Continue Reading

Citations

Jan 1, 1995·Virus Genes·E DomingoI Fita
Sep 1, 2001·Preventive Veterinary Medicine·D T HaydonN J Knowles
Jan 16, 2003·Virus Research·Esteban DomingoFrancisco Sobrino
Apr 16, 2004·Clinical Microbiology Reviews·Marvin J Grubman, Barry Baxt
Nov 22, 2000·The Veterinary Quarterly·P Moonen, R Schrijver
Oct 23, 2012·Biologicals : Journal of the International Association of Biological Standardization·K U AnilR Venkataramanan
Sep 29, 2011·Virus Research·Samuel OjosnegrosEsteban Domingo
Mar 14, 2007·Virus Research·Esteban Domingo, Jordi Gomez
Jul 11, 1998·Journal of Virology·E BaranowskiE Domingo

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