Selection of antigens and development of prototype tests for point-of-care leprosy diagnosis.

Clinical and Vaccine Immunology : CVI
Malcolm S DuthieSteven G Reed

Abstract

Leprosy can be a devastating chronic infection that causes nerve function impairment and associated disfigurement. Despite the recent reduction in the number of registered worldwide leprosy cases as a result of the widespread use of multidrug therapy, the number of new cases detected each year remains relatively stable. The diagnosis of leprosy is currently based on the appearance of clinical signs and requires expert clinical, as well as labor-intensive and time-consuming laboratory or histological, evaluation. For the purpose of developing an effective, simple, rapid, and low-cost diagnostic alternative, we have analyzed the serologic antibody response to identify Mycobacterium leprae proteins that are recognized by leprosy patients. More than 100 recombinant antigens were analyzed in a protein array format to select those with discriminatory properties for leprosy diagnosis. As expected, multibacillary leprosy patients recognized more antigens with stronger antibody responses than paucibacillary leprosy patients. Our data indicate, however, that multibacillary patients can be distinguished from paucibacillary patients, and both of these groups can be segregated from endemic control groups. We went on to confirm the diagnosti...Continue Reading

References

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Sep 28, 2007·Clinical and Vaccine Immunology : CVI·Malcolm S DuthieSteven G Reed

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Citations

May 6, 2011·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M S DuthieS G Reed
Nov 15, 2011·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·L H SampaioM S Duthie
Mar 13, 2014·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·R M OliveiraM M A Stefani
Jun 8, 2014·Transactions of the Royal Society of Tropical Medicine and Hygiene·Marilena Maria de SouzaMalcolm S Duthie
Mar 30, 2013·Expert Opinion on Medical Diagnostics·Annemieke Geluk
Jan 11, 2012·Scandinavian Journal of Immunology·O Parkash, B P Singh
Aug 4, 2015·Diagnostic Microbiology and Infectious Disease·Aline Araújo FreitasMariane Martins Araújo Stefani
Dec 18, 2013·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Jillian M CappelloThomas Ippolito
Sep 12, 2014·PLoS Neglected Tropical Diseases·Deanna A HaggeLinda B Adams
Aug 11, 2016·Diagnostic Microbiology and Infectious Disease·Aline Araújo FreitasMariane Martins Araújo Stefani
Aug 2, 2012·Journal of Bioinformatics and Computational Biology·William KrivanDarrick Carter
May 19, 2016·Revista Da Sociedade Brasileira De Medicina Tropical·Angélica da Conceição Oliveira Coelho FabriFrancisco Carlos Félix Lana
Nov 13, 2015·Memórias do Instituto Oswaldo Cruz·Danielle de Freitas MizogutiMariane Martins Araújo Stefani
Jan 11, 2013·Memórias do Instituto Oswaldo Cruz·Emerith Mayra HungriaMariane Martins de Araújo Stefani
Dec 18, 2009·Clinical and Vaccine Immunology : CVI·Malcolm S DuthieDarrick Carter
Sep 14, 2017·Memórias do Instituto Oswaldo Cruz·Ana Paula Mendes CarvalhoFrancisco Carlos Félix Lana
Nov 27, 2020·International Reviews of Immunology·Luis Alberto Ribeiro FroesMirian Nacagami Sotto
Jan 20, 2020·Diagnostic Microbiology and Infectious Disease·Malcolm S DuthieMarivic F Balagon

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