Selection of aptamers against pathogenic bacteria and their diagnostics application

World Journal of Microbiology & Biotechnology
Lijun WangYanbin Li

Abstract

Aptamers are short nucleotide sequences which can specifically bind to a variety of targets with high affinity. They are identified and selected via systematic evolution of ligands by exponential enrichment (SELEX). Compared to antibodies, aptamers offer several advantages including easy labeling, high stability and lower cost. Those advantages make it possible to be a potential for use as a recognition probe to replace antibody in the diagnostic field. This article is intended to provide a comprehensive review, which is focused on systemizing recent advancements concerning SELEX procedures, with special emphasis on the key steps in SELEX procedures. The principles of various aptamer-based detections of pathogenic bacteria and their application are discussed in detail, including colorimetric detection, fluorescence detection, electrochemical detection, lateral flow strip test, mass sensitive detection and PCR-based aptasensor. By discussing recent research and future trends based on many excellent publications and reviews, we attempt to give the readers a comprehensive view in the field of aptamer selection against pathogenic bacteria and their diagnostics application. Authors hope that this review will promote lively and valua...Continue Reading

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Citations

Feb 5, 2019·Critical Reviews in Food Science and Nutrition·Caleb AcquahMichael Kobina Danquah
Aug 14, 2020·PloS One·Boutheina MarnissiIssam Hmila
Apr 26, 2020·World Journal of Microbiology & Biotechnology·Samet UcakVeli C Ozalp
Jul 8, 2020·International Journal of Molecular Sciences·Jan P ElskensAnnemieke Madder
Feb 9, 2021·World Journal of Microbiology & Biotechnology·Danliang LiLili Chen
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ousama Al ShanaaMarina Padkina

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Methods Mentioned

BETA
SELEX
biosensors
ELISA
PCR
electrophoresis
FRET
biosensor
surface
quartz crystal microbalance
chip

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