Selection of candidate probionts by two different screening strategies from Atlantic cod (Gadus morhua L.) larvae

Veterinary Microbiology
Anders Jón FjellheimO Vadstein

Abstract

Two primary selection criteria were used to collect a pool of nearly 500 candidate probiotic bacteria from Atlantic cod (Gadus morhua L.) larvae, i.e. the dominant intestinal bacterial flora and isolates with antagonistic activity against Vibrio anguillarum. Bacteria were isolated from cod larvae from five rearing groups with variable rearing technologies. The bacteria were brought to pure culture and characterized phenotypically. Based on properties such as uniqueness, dominance and fermentative ability, a selection of approximately 10% of the isolates were chosen from the initial pool of bacteria to reduce the number of candidates. These 55 isolates were characterized further in vitro regarding antagonism, adhesion to mucus, growth in mucus, production of extracellular enzymes, fish bile resistance and haemolytic properties. Based on the results of the in vitro tests, the number of probiotic candidates was further reduced to seven isolates. To evaluate the probiotic potential and to assure that the seven isolates were not harmful to the host, yolk sac larvae of cod were exposed to the isolates in a small-scale in vivo experiment. The in vivo experiment excluded two of the candidate bacteria due to increased mortality of cod l...Continue Reading

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Citations

Oct 22, 2011·Diseases of Aquatic Organisms·M T Carbajal-GonzálezJ M Aller-Gancedo
Jun 30, 2015·Journal of Applied Microbiology·N V Hai
Nov 29, 2017·Probiotics and Antimicrobial Proteins·Sekar JinendiranGopal Selvakumar
Jan 26, 2011·Antimicrobial Agents and Chemotherapy·Cisse Hedegaard PorsbyLone Gram
Apr 2, 2017·Applied and Environmental Microbiology·Gary P RichardsJohnna P Fay
Sep 14, 2018·Frontiers in Microbiology·Olav VadsteinPeter Bossier
Jun 25, 2021·Probiotics and Antimicrobial Proteins·Krishnaveni GovindarajRamesh Uthandakalaipandian
Aug 4, 2021·The ISME Journal·Jiasui LiSuhelen Egan
Aug 8, 2021·Animals : an Open Access Journal From MDPI·Ana Gutiérrez FalcónJosé Luís Martín-Barrasa

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