Selection of CD4+CD8+ thymocytes by complex formation with medulla-derived epithelial cells

Cellular Immunology
P Hugo, E F Potworowski

Abstract

The role of lymphostromal complexes in T-cell differentiation is far from elucidated, mainly because a clear association of a particular stromal cell type with a distinct thymocyte subset has never been identified. Using an in vitro system, detecting the adherence of thymocytes to a thymic medullary epithelial cell line (E-5), we showed that the phenotype of these thymocytes was that of cortical type: Thy-1hi, LFA-1+, PNAhi, CD4+CD8+, MEL-14-/lo, IL-2R-, CD3-/lo, and TcR V beta 8-/lo. They were enriched in cells in G2/M at the time of complex formation, showed a higher basal proliferation in culture, and did not respond to PHA, IL-2 and only marginally to Con A. These data show that complex formation with mouse thymic medullary epithelium selects for CD4+CD8+ thymocytes, as shown by the marked decrease in CD4+CD8-/CD4-CD8+ thymocytes, and the incapacity of CD4-CD8- thymocytes to adhere.

References

Sep 1, 1986·International Journal of Cell Cloning·P C Keng
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·O LeoJ A Bluestone
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K H SingerB F Haynes

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Citations

Sep 1, 1993·Immunology Today·R L BoydP Hugo
Feb 1, 1991·Immunology Today·R L Boyd, P Hugo
Dec 1, 1990·European Journal of Immunology·C CoutureE F Potworowski

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