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Selection of chromosomal DNA libraries using a multiplex CRISPR system in Saccharomyces cerevisiae

bioRxiv

Jul 21, 2014

Owen W RyanJamie H D Cate

Abstract

The directed evolution of biomolecules to improve or change their activity is central to many engineering and synthetic biology efforts. However, selecting improved variants from gene libraries in living cells requires plasmid expression systems that suffer from variable copy number eff...read more

Mentioned in this Paper

Biochemical Pathway
DNA Insertion Binding
Physiological Aspects
Plasmids
Saccharomyces cerevisiae
Yeasts
Mutagenesis Procedure
Biological Markers
Clustered Regularly Interspaced Short Palindromic Repeats
Cellobiose
8
16
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Selection of chromosomal DNA libraries using a multiplex CRISPR system in Saccharomyces cerevisiae

bioRxiv

Jul 21, 2014

Owen W RyanJamie H D Cate

PMID: 990007310

DOI: 10.1101/007310

Abstract

The directed evolution of biomolecules to improve or change their activity is central to many engineering and synthetic biology efforts. However, selecting improved variants from gene libraries in living cells requires plasmid expression systems that suffer from variable copy number eff...read more

Mentioned in this Paper

Biochemical Pathway
DNA Insertion Binding
Physiological Aspects
Plasmids
Saccharomyces cerevisiae
Yeasts
Mutagenesis Procedure
Biological Markers
Clustered Regularly Interspaced Short Palindromic Repeats
Cellobiose
8
16

Similar Papers Found In These Feeds

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