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Selection of chromosomal DNA libraries using a multiplex CRISPR system in Saccharomyces cerevisiae

bioRxiv

Jul 21, 2014

Owen W RyanJamie H D Cate

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Abstract

The directed evolution of biomolecules to improve or change their activity is central to many engineering and synthetic biology efforts. However, selecting improved variants from gene libraries in living cells requires plasmid expression systems that suffer from variable copy number eff...read more

Mentioned in this Paper

Mutagenesis Process
Saccharomyces cerevisiae allergenic extract
Genome
Physiological Aspects
Biochemical Pathway
Directed Evolution
Tissue Engineering
Membrane Transport Proteins
Research Study
DNA Library
8
16
Paper Details
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  • Selection of chromosomal DNA libraries using a multiplex CRISPR system in Saccharomyces cerevisiae

    bioRxiv

    Jul 21, 2014

    Owen W RyanJamie H D Cate

    PMID: 990007310

    DOI: 10.1101/007310

    Abstract

    The directed evolution of biomolecules to improve or change their activity is central to many engineering and synthetic biology efforts. However, selecting improved variants from gene libraries in living cells requires plasmid expression systems that suffer from variable copy number eff...read more

    Mentioned in this Paper

    Mutagenesis Process
    Saccharomyces cerevisiae allergenic extract
    Genome
    Physiological Aspects
    Biochemical Pathway
    8
    16
    Paper Details
    References
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