Feb 16, 2011

Selection of DNA aptamers recognizing small cell lung cancer using living cell-SELEX

The Analyst
Takao KuniiEiry Kobatake

Abstract

We applied Systematic Evolution of Ligands by EXponential enrichment using Small Cell Lung Cancer (SCLC) cells. A DNA aptamer was identified and evaluated by fluorescent confocal microscopy and flow cytometry. Our results showed that the DNA aptamer binds to molecules that exist predominantly on target SCLC cell surfaces compared with other types of SCLC cells.

  • References13
  • Citations42

References

  • References13
  • Citations42

Citations

Mentioned in this Paper

Aptamers, Nucleotide
Flow Cytometry
DNA Aptamers
Small Cell Carcinoma of Lung
Selex Aptamer Technique
Fluorescent stain
Fluorescein (5 or 6)-Isothiocyanate
Microscopy, Confocal
Carcinoma, Small Cell
Binding (Molecular Function)

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