Selection of Escherichia coli K-12 chromosomal mutants that prevent expression of F-plasmid functions.

Journal of Bacteriology
P M SilvermanR Birchman

Abstract

Chromosomal mutants of Escherichia coli deficient in the expression of F-plasmid functions were selected by mutagenizing F- cells, introducing an F' plasmid into the mutagenized cells by conjugation, and identifying transconjugants resistant to the donor-specific bacteriophage Q beta by a simple spray test. All but 1 of 25 mutants were defective in an extracellular stage of Q beta infection, suggesting that they fail to elaborate F-pili. At least six of these were also deficient as deoxyribonucleic acid donors. More than half of the mutants appear to be altered in peviously undetected chromosomal genes required for the expression of F-related cellular functions.

References

Feb 1, 1979·Journal of Bacteriology·T J Lerner, N D Zinder
Jan 1, 1979·Annual Review of Biochemistry·T Blumenthal, G G Carmichael
Mar 1, 1976·Bacteriological Reviews·B J BachmannA L Taylor
Oct 5, 1975·Journal of Molecular Biology·N KlecknerD Botstein
Dec 1, 1972·Bacteriological Reviews·B J Bachmann
Dec 1, 1972·Bacteriological Reviews·K B Low
May 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·B Low
May 5, 1967·Biochemical and Biophysical Research Communications·P M SilvermanR C Valentine

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Citations

Apr 1, 1982·Journal of Bacteriology·P M Silverman
Jun 1, 1994·Microbiological Reviews·L S FrostR A Skurray
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn

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