Selection of neural differentiation-specific genes by comparing profiles of random differentiation

Stem Cells
Min Su LeeWoong-Yang Park

Abstract

Differentiation of embryonic stem cells (ESCs) into neurons requires a high level of transcriptional regulation. To further understand the transcriptional regulation of neural differentiation of ESCs, we used oligonucleotide microarray to examine the gene expressions of the guided differentiation (GD) model for dopaminergic (DA) neurons from mouse ESCs. We also determined the gene expression profiles of the random differentiation (RD) model of mouse ESCs into embryoid bodies. From K-means clustering analysis using the expression patterns of the two models, most of the genes (1,282 of 1,884 genes [68.0%]) overlapped in their expression patterns. Six hundred twenty-two differentially expressed genes (DEGs) from the GD model by random variance F-test were classified by their critical molecular functions in neurogenesis and DNA replication (Gene Ontology analysis). However, 400 genes among GD-DEGs (64.3%) showed a high correlation with RD in Spearman's correlation analysis (Spearman's coefficient p(s) >or= .6). The genes showing marginal correlation (-.4 < p(s) < .6) were present in the early stages of differentiation of both GD and RD, which were non-specific to brain development. Finally, we distinguished 66 GD-specific genes bas...Continue Reading

References

Apr 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·G R Martin, M J Evans
Mar 1, 1968·Biometrika·M B Wilk, R Gnanadesikan
Aug 1, 1982·The Journal of Cell Biology·E M Jones-VilleneuveV I Kalnins
Mar 20, 1997·Biochimica Et Biophysica Acta·J Y ZhengM Kakinuma
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·O Saucedo-CardenasO M Conneely
Aug 1, 2000·The Journal of Biological Chemistry·K L FergusonR S Slack
Oct 13, 2000·The European Journal of Neuroscience·S A PrzyborskiP W Andrews
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·S R BornsteinS Roffler-Tarlov
Dec 29, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S ReppeK M Gautvik
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·M JiangL Birnbaumer
Jan 5, 2002·Differentiation; Research in Biological Diversity·J Czyz, A Wobus
Dec 6, 2002·Oncogene·Woong-Yang ParkJeong-Sun Seo
Aug 22, 2003·Organic & Biomolecular Chemistry·Caridad FernándezAlfonso Fernández-Mayoralas
Sep 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Marat V Avshalumov, Margaret E Rice
Apr 20, 2004·Current Topics in Developmental Biology·Taiping Chen, En Li
Nov 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Theresa M Cabrera-VeraHeidi E Hamm
Nov 13, 2004·Stem Cells·Xianmin ZengWilliam J Freed
Feb 18, 2005·Journal of Neurochemistry·Chang-Hwan ParkSang-Hun Lee

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Citations

Jun 25, 2013·Cellular and Molecular Neurobiology·Xing-Gang MaoXiang Zhang
Jul 12, 2012·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Lisa D MooreGuoping Fan
May 27, 2010·PLoS Biology·Christophe HeinrichBenedikt Berninger
Dec 17, 2009·Journal of Preventive Medicine and Public Health = Yebang Ŭihakhoe Chi·Kyoung-Mu LeeDaehee Kang
Jul 19, 2012·Cellular and Molecular Life Sciences : CMLS·Guido Hansen, Rolf Hilgenfeld
Nov 19, 2008·International Journal of Radiation Biology·Tai-Qin HuangWoong-Yang Park
Sep 30, 2008·International Journal of Radiation Biology·Tai-Qin HuangWoong-Yang Park
Mar 24, 2016·Cell Biochemistry and Function·Do-Yeon Kim
Nov 12, 2015·Frontiers in Neuroscience·Patrick M HechtDaniel B Campbell
Oct 11, 2020·Biomedicines·Mikhail SavitskyVladimir L Katanaev
Jul 3, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Do-Hyun NamWoong-Yang Park

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