PMID: 9661811Jul 14, 1998Paper

Selection of phage displayed peptides from a random 10-mer library recognising a peptide target

Immunotechnology : an International Journal of Immunological Engineering
T BremnesOddmund Bakke

Abstract

Peptide display libraries are powerful tools in the search for detailed information about protein-protein interactions. Usual targets for isolation of phage displayed peptide ligands include antibodies, various receptors, other full size proteins or larger fragments thereof. Smaller protein fragments such as synthetic peptides have not been reported as targets for screening of peptide display libraries. To investigate whether a protein target used for screening of a peptide display library could be scaled down to peptide size. As the peptide target we wanted to use a sequence derived from the cytosolic tail of MHC class II associated invariant chain containing a leucine class endosomal sorting signal, known to be recognised as an autonomous functional unit during targeting of class II complexes to antigen processing compartments. A screening procedure where a synthetic 15-mer invariant chain peptide was coupled to a methacrylate matrix of high binding capacity was developed, and three rounds of selection were performed from a random 10-mer fUSE5 display library. The peptide display library was successfully enriched for phage clones with affinity for the invariant chain peptide. Furthermore, the binding phage clones were able to...Continue Reading

References

Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W R Pearson, D J Lipman
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·F R DeLeoM T Quinn
Aug 25, 1995·The Journal of Biological Chemistry·L PondP A Peterson
Jan 1, 1993·Methods in Enzymology·G P Smith, J K Scott
Jul 30, 1993·Science·A Sorkin, G Carpenter
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·S Y LeeY Choi
Dec 1, 1996·Current Opinion in Biotechnology·R CorteseF Felici
Aug 1, 1994·Trends in Cell Biology·I V Sandoval, O Bakke

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Citations

Aug 17, 1999·Current Opinion in Structural Biology·R W Roberts, W W Ja
Apr 9, 2005·The Journal of Peptide Research : Official Journal of the American Peptide Society·N ZhouY F Zhao
Mar 25, 2005·Environment International·S W ZhaoZ C Hua
Feb 3, 2004·Journal of Receptor and Signal Transduction Research·Michael Szardenings

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