Selection of potent inhibitors of farnesyl-protein transferase from a synthetic tetrapeptide combinatorial library.

The Journal of Biological Chemistry
A WallaceA Pessi

Abstract

Inhibitors of farnesyl-protein transferase (FPTase) show promise as anticancer agents. Based on the sequence of the protein substrates of FPTase (the CAAX sequence), potent and selective peptidomimetic inhibitors have been developed; these compounds share with the peptide substrate a free thiol and a C-terminal carboxylate. We have used a synthetic tetrapeptide combinatorial library to screen for new leads devoid of these features: the peptides were C-terminally amidated, and no free thiol was included in the combinatorial building blocks. To compensate for this negative bias, an expanded set of 68 amino acids was used, including both L and D as well as many non-coded residues. Sixteen individual tetrapeptides derived from the consensus were synthesized and tested; all were active, showing IC50 values ranging from low micromolar to low nanomolar. The most active peptide, D-tryptophan-D-methionine-D-4-chlorophenylalanine-L-gamma- carboxyglutamic acid (Ki = 2 nM), is also very selective showing little inhibitory activity against the related enzyme geranylgeranyl-protein transferase type I (IC50 > 50 microM). In contrast to CAAX-based peptidomimetics, D-tryptophan-D-methionine-D-4-chlorophenylalanine-L-gamma-carboxyglut amic acid ...Continue Reading

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Citations

Jan 13, 1998·Current Opinion in Biotechnology·P L Myers
Apr 17, 1999·Bioorganic & Medicinal Chemistry Letters·R LiuC Scolastico
Aug 1, 1997·Current Opinion in Chemical Biology·J B GibbsA Oliff
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Oct 3, 1999·Journal of Medicinal Chemistry·R A HoughtenJ M Ostresh

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