Selection of reference genes for real-time quantitative reverse transcription-polymerase chain reaction in hippocampal structure in a murine model of temporal lobe epilepsy with focal seizures

Journal of Neuroscience Research
Fabien PernotAndré Peinnequin

Abstract

Reference genes are often used to normalize expression of data from real-time quantitative reverse transcription-polymerase chain reaction (RT-qPCR), and only a validation of their stability during a given experimental paradigm leads to reliable interpretations. The present study was thus designed to validate potential reference genes in a mouse model of mesiotemporal lobe epilepsy (MTLE) with focal seizures after unilateral intrahippocampal injection of kainate (KA). Ipsilateral and contralateral hippocampi were removed during nonconvulsive status epilepticus (5 hr), epileptogenesis (7 days), and the chronic period of recurrent focal seizures (21 days). Naive animals were equally studied. The stability of eight potential reference genes (hypoxanthine phosphoribosyltransferase, Hprt1; peptidylprolyl isomerase A, Ppia; TATA box binding protein, Tbp; beta-actin, Actb; acidic ribosomal phosphoprotein P0, Arbp; glyceraldehyde-3-phosphate dehydrogenase, Gapdh; ribosomal RNA 18S, 18S rRNA; and glucuronidase beta, Gusb) were determined using geNorm and NormFinder software. The first five (Hprt1, Ppia, Tbp, Actb, and Arbp) were found to be stable across the different phases of the disease and appeared adequate for normalizing RT-qPCR d...Continue Reading

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Citations

Jan 14, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jie ChenHiroshi Nishimune
Jun 4, 2013·Autonomic Neuroscience : Basic & Clinical·Chloe Stenkamp-StrahmOnesmo Balemba
Nov 18, 2018·Brain Pathology·Alana HoffmannJürgen Winkler
Feb 1, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Paola Castro-GarciaCarmen Ramírez-Castillejo
Apr 10, 2017·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Leonardo PedrazzaJarbas Rodrigues de Oliveira

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