PMID: 4349028Apr 1, 1973

Selection of respiratory mutants of Neurospora crassa

Journal of Bacteriology
D L EdwardsJ Horstmann

Abstract

A method is described that permits the isolation of mutants that are defective in mitochondrial respiration. The techniques of inositol-less death and overlay with 2,3,5-triphenyl-2H-tetrazolium chloride are utilized to select for mutant colonies. Colonies that survive inositol-less death and fail to reduce the tetrazolium dye are then tested polarographically for cyanide-sensitive respiration. A preliminary characterization of three mutants obtained by this method is presented. The mutants have been characterized by their cyanide-sensitive respiration rate, growth rate, the state III respiration rate of isolated mitochondria, inhibition of the respiration of isolated mitochondria by cyanide and antimycin A, and cytochrome spectra. All of the mutants described differ from the parent strain in some of these aspects.

Citations

Feb 16, 1976·European Journal of Biochemistry·D L Edwards, E Rosenberg
Jun 24, 1977·Molecular & General Genetics : MGG·H BertrandC A Zagozeski
Jun 1, 1978·Molecular & General Genetics : MGG·F Ruiz, A Adoutte
Apr 17, 1978·European Journal of Biochemistry·D L EdwardsJ T Warden

Related Concepts

Antimycin A1
Centrifugation, Density Gradient
Isocyanides
Inositol
Study, Methodological
Mitochondria
NADH
Neurospora
Neurospora crassa
Oxygen Consumption

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